TY - JOUR
T1 - Identification of anaerobic arsenite-oxidizing and arsenate-reducing bacteria associated with an alkaline saline lake in Khovsgol, Mongolia
AU - Hamamura, Natsuko
AU - Itai, Takaaki
AU - Liu, Yitai
AU - Reysenbach, Anna Louise
AU - Damdinsuren, Narantuya
AU - Inskeep, William P.
N1 - Publisher Copyright:
© 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Microbial arsenic transformation pathways associated with a saline lake located in northern Mongolia were examined using molecular biological and culturing approaches. Bacterial 16S rRNA gene sequences recovered from saline lake sediments and soils were affiliated with haloalkaliphiles, including Bacillus and Halomonas spp. Diverse sequences of arsenate respiratory reductase (arrA) and a new group of arsenite oxidase (arxA) genes were also identified. Pure cultures of arsenate-reducing Nitrincola strain and anaerobic arsenite-oxidizing Halomonas strain were isolated. The chemoorganotrophic Halomonas strain contains arxA gene similar to that of a chemoautotrophic arsenite-oxidizing Alkalilimnicola ehrlichii strain MLHE-1. These results revealed the diversity of arsenic transformation pathways associated with a geographically distinct saline system and the potential contribution of arx-dependent arsenite oxidation by heterotrophic bacteria.
AB - Microbial arsenic transformation pathways associated with a saline lake located in northern Mongolia were examined using molecular biological and culturing approaches. Bacterial 16S rRNA gene sequences recovered from saline lake sediments and soils were affiliated with haloalkaliphiles, including Bacillus and Halomonas spp. Diverse sequences of arsenate respiratory reductase (arrA) and a new group of arsenite oxidase (arxA) genes were also identified. Pure cultures of arsenate-reducing Nitrincola strain and anaerobic arsenite-oxidizing Halomonas strain were isolated. The chemoorganotrophic Halomonas strain contains arxA gene similar to that of a chemoautotrophic arsenite-oxidizing Alkalilimnicola ehrlichii strain MLHE-1. These results revealed the diversity of arsenic transformation pathways associated with a geographically distinct saline system and the potential contribution of arx-dependent arsenite oxidation by heterotrophic bacteria.
UR - http://www.scopus.com/inward/record.url?scp=84907861944&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907861944&partnerID=8YFLogxK
U2 - 10.1111/1758-2229.12144
DO - 10.1111/1758-2229.12144
M3 - Article
C2 - 25646538
AN - SCOPUS:84907861944
SN - 1758-2229
VL - 6
SP - 476
EP - 482
JO - Environmental Microbiology Reports
JF - Environmental Microbiology Reports
IS - 5
ER -