TY - JOUR
T1 - Identification of an epitope for T-cells correlated with antibody response to hepatitis B surface antigen in vaccinated humans
AU - Min, Wei Ping
AU - Kamikawaji, Nobuhiro
AU - Mineta, Mari
AU - Tana, Takeshi
AU - Kashiwagi, Seizaburo
AU - Sasazuki, Takehiko
N1 - Funding Information:
We thank the medical students at Kyushu University who provided blood samples many times, and the Chemo-SeroTherapy Research Institute for providing the affinity-purified HBsAg. We are grateful to Drs. Rui-ping Dong, Satoru Senju, and Akinori Kimura for their help, and to C. J. Savoie for reading the manuscript. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Japan, and by a researchg rant from the Ministry of Health and Welfare, Japan.
PY - 1996/4/1
Y1 - 1996/4/1
N2 - Two antigenic T-cell epitopes of HBsAg, designated HBs16-31 and HBs81-99, were identified using synthetic peptides and HBsAg-specific T-cell lines. HBs16-31 was recognized by five HBsAg-specific T-cell lines from vaccinees with both high and low antibody titers, whereas HBs81-99 was recognized by two T-cell lines derived from vaccinees with high antibody titers. The antibody titer against HBsAg was correlated significantly with the proliferation of vaccinee's PBLs in response to HBs81-99 (r = 0.47) but not to HBs16-31, suggesting that HBs81-99 plays a critical role in anti-HBs antibody production in humans vaccinated with HBsAg.
AB - Two antigenic T-cell epitopes of HBsAg, designated HBs16-31 and HBs81-99, were identified using synthetic peptides and HBsAg-specific T-cell lines. HBs16-31 was recognized by five HBsAg-specific T-cell lines from vaccinees with both high and low antibody titers, whereas HBs81-99 was recognized by two T-cell lines derived from vaccinees with high antibody titers. The antibody titer against HBsAg was correlated significantly with the proliferation of vaccinee's PBLs in response to HBs81-99 (r = 0.47) but not to HBs16-31, suggesting that HBs81-99 plays a critical role in anti-HBs antibody production in humans vaccinated with HBsAg.
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U2 - 10.1016/0198-8859(96)00009-2
DO - 10.1016/0198-8859(96)00009-2
M3 - Article
C2 - 8727207
AN - SCOPUS:0029998951
SN - 0198-8859
VL - 46
SP - 93
EP - 99
JO - Human Immunology
JF - Human Immunology
IS - 2
ER -
