TY - JOUR
T1 - Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER
AU - Shakoori, Abbas
AU - Fujii, Gourou
AU - Yoshimura, Shin Ichiro
AU - Kitamura, Masashi
AU - Nakayama, Kazuhisa
AU - Ito, Takashi
AU - Ohno, Hiroshi
AU - Nakamura, Nobuhiro
N1 - Funding Information:
We thank Drs. Kazuhisa Ota and Naoyuki Hayashi (Kanazawa University) for helpful comments and discussions, and Dr. Naotaka Tanaka (Kagawa University) for providing poly(A) + RNA from HeLa cells. This work was supported by Grant-in-Aid for Scientific Research (12680688, 15570156) and Grant-in-Aid for Scientific Research on Priority Areas (13216040, 14037221, and 15032216) from The Ministry of Education, Culture, Sports, Science and Technology of Japan for N.N. S.Y. was supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2003/12/19
Y1 - 2003/12/19
N2 - A family of five-pass transmembrane proteins (FinGERs) were identified from the protein sequence database. The family includes yeast Yip1p, Yip4p, Yip5p, and Yif1p, and also their plant, insects, nematode, and mammalian homologues, suggesting their conserved function in a broad range of species. Eight family members were found in human. Multiple sequence alignment revealed three regions conserved among all family members. All of the human family members were expressed widely in various tissues. The human proteins were localized in and around the Golgi apparatus and may also be in the ER to some extent. The Golgi apparatus was fragmented by overexpression of the five of the family members. Some of the members were found to interact by yeast two-hybrid analysis, suggesting the formation of a complex. These results suggest that FinGERs function in maintenance of the Golgi structure and/or transport between the ER and the Golgi apparatus.
AB - A family of five-pass transmembrane proteins (FinGERs) were identified from the protein sequence database. The family includes yeast Yip1p, Yip4p, Yip5p, and Yif1p, and also their plant, insects, nematode, and mammalian homologues, suggesting their conserved function in a broad range of species. Eight family members were found in human. Multiple sequence alignment revealed three regions conserved among all family members. All of the human family members were expressed widely in various tissues. The human proteins were localized in and around the Golgi apparatus and may also be in the ER to some extent. The Golgi apparatus was fragmented by overexpression of the five of the family members. Some of the members were found to interact by yeast two-hybrid analysis, suggesting the formation of a complex. These results suggest that FinGERs function in maintenance of the Golgi structure and/or transport between the ER and the Golgi apparatus.
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U2 - 10.1016/j.bbrc.2003.10.197
DO - 10.1016/j.bbrc.2003.10.197
M3 - Article
C2 - 14680843
AN - SCOPUS:0344552816
SN - 0006-291X
VL - 312
SP - 850
EP - 857
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -