TY - JOUR
T1 - Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium
AU - Himeno, Kohei
AU - Rosengren, K. Johan
AU - Inoue, Tomoko
AU - Perez, Rodney H.
AU - Colgrave, Michelle L.
AU - Lee, Han Siean
AU - Chan, Lai Y.
AU - Henriques, Sónia Troeira
AU - Fujita, Koji
AU - Ishibashi, Naoki
AU - Zendo, Takeshi
AU - Wilaipun, Pongtep
AU - Nakayama, Jiro
AU - Leelawatcharamas, Vichien
AU - Jikuya, Hiroyuki
AU - Craik, David J.
AU - Sonomoto, Kenji
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/8/11
Y1 - 2015/8/11
N2 - Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu24 and Trp87 to yield the mature (circular) enterocin NKR-5-3B.
AB - Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu24 and Trp87 to yield the mature (circular) enterocin NKR-5-3B.
UR - http://www.scopus.com/inward/record.url?scp=84938703013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938703013&partnerID=8YFLogxK
U2 - 10.1021/acs.biochem.5b00196
DO - 10.1021/acs.biochem.5b00196
M3 - Article
C2 - 26174911
AN - SCOPUS:84938703013
SN - 0006-2960
VL - 54
SP - 4863
EP - 4876
JO - Biochemistry
JF - Biochemistry
IS - 31
ER -