TY - JOUR
T1 - Identification and characterization of the fusion transcript, composed of the apterous homolog and a putative protein phosphatase gene, generated by 1.5-Mb interstitial deletion in the vestigial (Vg) mutant of Bombyx mori
AU - Fujii, T.
AU - Abe, H.
AU - Katsuma, S.
AU - Shimada, T.
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research ( No. 22128004 ), Professional Program for Agricultural Bioinformatics (MEXT) , and the Agrigenome Research Program (MAFF-NIAS), Japan . The silkworm strains and DNA clones were provided by the National BioResource Project (MEXT), Japan. We also thank the members of Institute for Sustainable Agro-ecosystem Services, University of Tokyo, for their supports in mulberry cultivation.
PY - 2011/5
Y1 - 2011/5
N2 - The vestigial (Vg) mutant is a Z-linked mutant that causes vestigial wings in the silkworm, Bombyx mori. We have previously reported a 1.5-Mb interstitial deletion on the Z chromosome bearing the Vg mutation (ZVg chromosome). In this study, we found that exons 3-8 of a gene named Bmptp-Z encoding a putative tyrosine-specific protein phosphatase are deleted by the 1.5-Mb interstitial deletion. We found that a gene encoding the Bombyx homolog of Drosophila Apterous (BmAp-A) protein is located 4.5 kb downstream of the distal breakpoint of the 1.5-Mb interstitial deletion. Moreover, an in-frame fusion transcript composed of the 5' part of Bmptp-Z and the 3' part of Bmap-A is generated specific to the ZVg chromosome. Effects of the in-frame fusion transcript on the vestigial phenotype are discussed.
AB - The vestigial (Vg) mutant is a Z-linked mutant that causes vestigial wings in the silkworm, Bombyx mori. We have previously reported a 1.5-Mb interstitial deletion on the Z chromosome bearing the Vg mutation (ZVg chromosome). In this study, we found that exons 3-8 of a gene named Bmptp-Z encoding a putative tyrosine-specific protein phosphatase are deleted by the 1.5-Mb interstitial deletion. We found that a gene encoding the Bombyx homolog of Drosophila Apterous (BmAp-A) protein is located 4.5 kb downstream of the distal breakpoint of the 1.5-Mb interstitial deletion. Moreover, an in-frame fusion transcript composed of the 5' part of Bmptp-Z and the 3' part of Bmap-A is generated specific to the ZVg chromosome. Effects of the in-frame fusion transcript on the vestigial phenotype are discussed.
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U2 - 10.1016/j.ibmb.2011.01.007
DO - 10.1016/j.ibmb.2011.01.007
M3 - Article
C2 - 21296154
AN - SCOPUS:79953031020
SN - 0965-1748
VL - 41
SP - 306
EP - 312
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 5
ER -