Hypotonic stress-induced NO production in endothelium depends on endogenous ATP

Chiwaka Kimura, Tetsuya Koyama, Masahiro Oike, Yushi Ito

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


The mechanism by which mechanical stress induces nitric oxide (NO) synthesis in endothelium is still controversial. Hypotonic stress (HTS, -20%) induced ATP release, which evoked Ca2+ transients in bovine aortic endothelial cells (BAEC). HTS also induced NO synthesis, assessed by DAF-2 fluorescence, which was suppressed by inhibiting endogenous ATP-induced Ca2+ transients with suramin or neomycin. Exogenously applied ATP mimicked these responses. Pretreatment with wortmannin did not affect DAF-2 fluorescence, suggesting that Akt phosphorylation was not involved in HTS-induced NO synthesis. These results indicate that endogenous ATP plays a central role in HTS-induced NO synthesis in BAEC. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)736-740
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - Aug 11 2000

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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