Hybrid chitosan membranes tested in sheep for guided tissue regeneration

P. P. Cortez, Y. Shirosaki, C. M. Botelho, M. J. Simões, F. Gartner, R. M.Gil Da Costa, K. Tsuru, S. Hayakawa, A. Osaka, M. A. Lopes, J. D. Santos, A. C. Maurício

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Previous in vitro studies confirmed an improved cytocompatibility of chitosan-silicate hybrid membranes over chitosan membranes. The main goal of this study was to assess the in vivo histocompatibility of both membranes through subcutaneous implantations at different time periods, 1 week, 1, 2 and 3 months, using a sheep model. Chitosan membranes elicited an exuberant inflammatory response and were consequently rejected. The hybrid chitosan membranes were not rejected and the degree of inflammatory response decreased gradually until the third month of implantation. Histological evaluation also showed that these membranes can be resorbed in vivo. This study demonstrates that the incorporation of silicate into the chitosan solution improves its histocompatibility, indicating that the hybrid chitosan-silicate membranes are suitable candidates to be used in clinical applications.

Original languageEnglish
Pages (from-to)1265-1268
Number of pages4
JournalKey Engineering Materials
Volume361-363 II
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • Mechanics of Materials
  • Mechanical Engineering


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