HPLC analysis of naturally occurring free d-amino acids in mammals

Yurika Miyoshi, Reiko Koga, Tsubasa Oyama, Hai Han, Kyoko Ueno, Kei Masuyama, Yusuke Itoh, Kenji Hamase

Research output: Contribution to journalReview articlepeer-review

86 Citations (Scopus)


d-Amino acids are currently recognized as naturally occurring physiologically active substances and biomarkers in mammals. The progress of analytical technologies, mostly high resolution chromatographic or electrodriven separation methods, has significantly contributed to the advances in d-amino acid research in real biological matrices. In this review, we would like to describe the d-amino acid research, from the discovery of appreciable amounts of free d-amino acids in mammals to the current metabolomics study focusing on amino acid enantiomers. The liquid phase enantioselective analytical methods utilized for the determination of d-amino acids in mammals including human beings will be discussed.

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Publication statusPublished - Oct 2012

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry


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