TY - JOUR
T1 - Homogeneous bioassays based on the manipulation of magnetic nanoparticles by rotating and alternating magnetic FieldsA comparison
AU - Dieckhoff, Jan Henrik
AU - Yoshida, Takashi
AU - Enpuku, Keiji
AU - Schilling, Meinhard
AU - Ludwig, Frank
N1 - Funding Information:
This work was supported by the European Commission Framework Programme 7 under the NAMDIATREAM project (NMP-2010-246479) and by a Grant-in-Aid for Young Scientists (B) from JSPS (23760369).
PY - 2012
Y1 - 2012
N2 - The rotating magnetic field as a source of magnetic nanoparticle manipulation for the use in homogenous bioassays is presented and compared with the frequently used alternating magnetic field technique. For the investigation of the impact of the rotating and alternating field mode on the biomolecule detection, a fluxgate based measurement system has been used. This system detects the MNPs magnetization stray field and calculates the phase lag between the aligning field and the MNPs magnetization. By analyzing the phase lag, the analysis is not altered by changes in MNP concentration. The measured phase lag spectra show a significant difference between both magnetic field modes and agree well with simulations based on the Fokker-Planck equation. A modeling of binding experiments based on these simulations predicts a higher sensitivity for the rotating magnetic field manipulation.
AB - The rotating magnetic field as a source of magnetic nanoparticle manipulation for the use in homogenous bioassays is presented and compared with the frequently used alternating magnetic field technique. For the investigation of the impact of the rotating and alternating field mode on the biomolecule detection, a fluxgate based measurement system has been used. This system detects the MNPs magnetization stray field and calculates the phase lag between the aligning field and the MNPs magnetization. By analyzing the phase lag, the analysis is not altered by changes in MNP concentration. The measured phase lag spectra show a significant difference between both magnetic field modes and agree well with simulations based on the Fokker-Planck equation. A modeling of binding experiments based on these simulations predicts a higher sensitivity for the rotating magnetic field manipulation.
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U2 - 10.1109/TMAG.2012.2198797
DO - 10.1109/TMAG.2012.2198797
M3 - Article
AN - SCOPUS:84867760771
SN - 0018-9464
VL - 48
SP - 3792
EP - 3795
JO - IEEE Transactions on Magnetics
JF - IEEE Transactions on Magnetics
IS - 11
M1 - 6332681
ER -