TY - JOUR
T1 - Homogeneity of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus
AU - Hiromasa, Yasuaki
AU - Aso, Yoichi
AU - Yamashita, Shoji
AU - Aikawa, Yoshikatsu
PY - 1995/3
Y1 - 1995/3
N2 - The pyruvate dehydrogenase multienzyme complex was purified from Bacillus stearothermophilus by means of six gel-filtration column chromatographies; once on Cellulofine GCL-2000, twice on Sepharose CL-2B, and three times on Sephacryl S-500HR. The molecular size distribution of the complex was examined in detail by gel-filtration chromatography, analytical and sucrose-density ultracentrifugations, and dynamic light scattering. The complex was found to be homogeneous; a dimeric complex was undetectable even with a high concentration of protein (below 6.8 mg/ml).
AB - The pyruvate dehydrogenase multienzyme complex was purified from Bacillus stearothermophilus by means of six gel-filtration column chromatographies; once on Cellulofine GCL-2000, twice on Sepharose CL-2B, and three times on Sephacryl S-500HR. The molecular size distribution of the complex was examined in detail by gel-filtration chromatography, analytical and sucrose-density ultracentrifugations, and dynamic light scattering. The complex was found to be homogeneous; a dimeric complex was undetectable even with a high concentration of protein (below 6.8 mg/ml).
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U2 - 10.1093/oxfordjournals.jbchem.a124730
DO - 10.1093/oxfordjournals.jbchem.a124730
M3 - Article
C2 - 7629008
AN - SCOPUS:0028964805
SN - 0021-924X
VL - 117
SP - 467
EP - 470
JO - Journal of biochemistry
JF - Journal of biochemistry
IS - 3
ER -