HLA-DR2-restricted responses to proteolipid protein 95-116 peptide cause autoimmune encephalitis in transgenic mice

Kazuyuki Kawamura, Takashi Yamamura, Kazumasa Yokoyama, De Hua Chui, Yoshinori Fukui, Takehiko Sasazuki, Hidetoshi Inoko, Chella S. David, Takeshi Tabira

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)


In multiple sclerosis (MS) patients who carry the Class II major histocompatibility (MHC) type HLA-DR2, T cells specific for amino acids 95- 116 in the proteolipid protein (PLP) are activated and clonally expanded. However, it remains unclear whether these autoreactive T cells play a pathogenic role or, rather, protect against the central nervous system (CNS) damage. We have addressed this issue, using mice transgenic for the human MHC class II region carrying the HLA-DR2 (DRB1*1502) haplotype. After stimulating cultured lymph node cells repeatedly with PLP95-116, we generated 2 HLA-DR2-restricted, PLP95-116-specific T-cell lines (TCLs) from the transgenic mice immunized with this portion of PLP. The TCLs were CD4+ and produced T-helper 1 (Th1) cytokines in response to the peptide. These TCLs were adoptively transferred into RAG-2(-/-) mice expressing HLA-DR2 (DRB1*1502) molecules. Mice receiving 1 of the TCLs developed a neurological disorder manifested attic movement without apparent paresis on day 3, 4, or 5 after cell transfer. Histological examination revealed inflammatory loci primarily restricted to the cerebrum and cerebellum, in association with scattered demyelinating lesions in the deep cerebral cortex. These results support a pathogenic role for PLP95-116-specific T cells in HLA-DR2+ MS patients, and shed light on the possible correlation between autoimmune target epitope and disease phenotype in human CNS autoimmune diseases.

Original languageEnglish
Pages (from-to)977-984
Number of pages8
JournalJournal of Clinical Investigation
Issue number7
Publication statusPublished - Apr 2000

All Science Journal Classification (ASJC) codes

  • Medicine(all)


Dive into the research topics of 'HLA-DR2-restricted responses to proteolipid protein 95-116 peptide cause autoimmune encephalitis in transgenic mice'. Together they form a unique fingerprint.

Cite this