Highly sensitive surface plasmon resonance sensor on nanoscale bioactive surfaces for specific detection of tri-nitro toluene

Praveen Singh, Takeshi Onodera, Kiyoshi Matsumoto, Norio Miura, Kiyoshi Toko

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


A nano-scale biosensor chip surface was fabricated using dinitro-phenylated keyhole limpet hemocyanin (DNP-KLH) protein conjugate as ligand supported by underlying 11-amino 1-undecanethiol hydrochloride(AUT) self-assembled monolayer (SAM) and bis sulfosuccinimidyl suberate (BS3) as crosslinker, Bioactive thin films were fabricated over gold chip via layer-by-layer self- assembly methods. Biomolecular interaction between substrate specific anti-TNT antibody and DNP-KLH conjugate surface was monitored through surface plasmon resonance based optical sensor. The quantitation of tri-nitro toluene(TNT) on this bioactive surface was done using the solution based competitive inhibition assay. The DNP-KLH surface biosensor has shown a detection limit of 0.14 ng/ml (140 ppt) for TNT molecule. The detection limit of surface plasmon resonance (SPR) biosensor was further enhanced by using goat anti mouse antibody to the 0.002 ng/ml for TNT analyte. This TNT specific biosensor holds the promise to be one of most sensitive sensor surface under indirect competitive assay format, A short injection (12 sec) of 10 mM glycine-HCl solution was found adequate for regeneration of DNP-KLH surface for repeated use.

Original languageEnglish
Title of host publicationNanofunctional Materials, Nanostructures and Novel Devices for Biological and Chemical Detection
PublisherMaterials Research Society
Number of pages8
ISBN (Print)9781604234077
Publication statusPublished - 2006
Event2006 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 27 2006Dec 1 2006

Publication series

NameMaterials Research Society Symposium Proceedings
ISSN (Print)0272-9172


Other2006 MRS Fall Meeting
Country/TerritoryUnited States
CityBoston, MA

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering


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