TY - JOUR
T1 - High-throughput simultaneous analysis of pesticides by supercritical fluid chromatography/tandem mass spectrometry
AU - Ishibashi, Megumi
AU - Ando, Takashi
AU - Sakai, Miho
AU - Matsubara, Atsuki
AU - Uchikata, Takato
AU - Fukusaki, Eiichiro
AU - Bamba, Takeshi
PY - 2012/11/30
Y1 - 2012/11/30
N2 - Combination techniques such as gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) are commonly used for pesticide residue analysis, but there is no reported method for the simultaneous analysis of multiple pesticides in a sample using a single instrument. Supercritical fluid chromatography (SFC) offers high resolution at high flow rates and various separation modes and hence may aid the rapid simultaneous analysis of pesticide. We developed an SFC/MS/MS method and analyzed 17 pesticides with a wide range of polarities (logPow=-4.6 to 7.05) and molecular weights (112.1-888.6) within 11min using a polar-embedded reversed-phase column. To the best of our knowledge, there is no previous report on the SFC analysis of a wide variety of compounds, including highly hydrophilic ones. By SFC, diquat dibromide (logPow=-4.6), together with cypermethrin (logPow=6.6) and tralomethrin (logPow=5.05), could be detected in the presence of various other pesticides using a single mobile phase. SFC/MS allows for the rapid and simultaneous analysis of low concentrations (ng/L levels) of pesticides that typically need to be analyzed by GC/MS and LC/MS separately.
AB - Combination techniques such as gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) are commonly used for pesticide residue analysis, but there is no reported method for the simultaneous analysis of multiple pesticides in a sample using a single instrument. Supercritical fluid chromatography (SFC) offers high resolution at high flow rates and various separation modes and hence may aid the rapid simultaneous analysis of pesticide. We developed an SFC/MS/MS method and analyzed 17 pesticides with a wide range of polarities (logPow=-4.6 to 7.05) and molecular weights (112.1-888.6) within 11min using a polar-embedded reversed-phase column. To the best of our knowledge, there is no previous report on the SFC analysis of a wide variety of compounds, including highly hydrophilic ones. By SFC, diquat dibromide (logPow=-4.6), together with cypermethrin (logPow=6.6) and tralomethrin (logPow=5.05), could be detected in the presence of various other pesticides using a single mobile phase. SFC/MS allows for the rapid and simultaneous analysis of low concentrations (ng/L levels) of pesticides that typically need to be analyzed by GC/MS and LC/MS separately.
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U2 - 10.1016/j.chroma.2012.09.067
DO - 10.1016/j.chroma.2012.09.067
M3 - Article
C2 - 23102524
AN - SCOPUS:84868380483
SN - 0021-9673
VL - 1266
SP - 143
EP - 148
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -