TY - GEN
T1 - High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level
AU - Hattori, Atsunori
AU - Yasui, Takao
AU - Kaji, Noritada
AU - Baba, Yoshinobu
N1 - Funding Information:
This work was partly supported the Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), the JSPS Grant-in-Aid for Scientific Research (A) 24241050.
Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - We detected methylation sites on elongated lambda DNA molecules as high fluorescent distinct peaks via double collar imaging. We used methyl-CpG-binding domain 2 protein (MBD2p) combined with the quantum dots (QDs) for methylation imaging, and YOYO-1 for DNA imaging.
AB - We detected methylation sites on elongated lambda DNA molecules as high fluorescent distinct peaks via double collar imaging. We used methyl-CpG-binding domain 2 protein (MBD2p) combined with the quantum dots (QDs) for methylation imaging, and YOYO-1 for DNA imaging.
UR - http://www.scopus.com/inward/record.url?scp=84983354242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983354242&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983354242
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 861
EP - 863
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -