TY - JOUR
T1 - High-throughput and highly sensitive analysis method for polyisoprene in plants by pyrolysis-gas chromatography/mass spectrometry
AU - Takeno, Shinya
AU - Bamba, Takeshi
AU - Nakazawa, Yoshihisa
AU - Fukusaki, Eiichiro
AU - Okazawa, Atsushi
AU - Kobayashi, Akio
N1 - Funding Information:
This work was partly supported by the New Energy and Industrial Technology Development Organization (NEDO of Japan).
PY - 2010
Y1 - 2010
N2 - Natural polyisoprene is a biopolymer consisting of isoprene units (C 5H8) that is used commercially in household, medical, and industrial materials. For the management of natural polyisoprene production, the selection of high-yield polyisoprene-producing trees, and an understanding of polyisoprene biosynthesis, a high-throughput and highly sensitive screening method for the quantification of polyisoprene is required. In this study, we examined pyrolysates from polyisoprenes, polyprenols, carotenoids, ubiquinone (CoQ-10), and sterols by pyrolysis gas chromatography/mass spectrometry (PyGC/MS) and determined that the amounts of isoprene and limonene released from polyprenols and polyisoprenes were dependent upon their molecular weights. Based on these results, we developed a relative quantification method for polyisoprene in leaves by direct analysis of 1 mg of leaves using PyGC/MS. This novel quantification method eliminated extraction steps and can be used in the measurement of polyisoprene contents in Eucommia ulmoides and Hevea brasiliensis.
AB - Natural polyisoprene is a biopolymer consisting of isoprene units (C 5H8) that is used commercially in household, medical, and industrial materials. For the management of natural polyisoprene production, the selection of high-yield polyisoprene-producing trees, and an understanding of polyisoprene biosynthesis, a high-throughput and highly sensitive screening method for the quantification of polyisoprene is required. In this study, we examined pyrolysates from polyisoprenes, polyprenols, carotenoids, ubiquinone (CoQ-10), and sterols by pyrolysis gas chromatography/mass spectrometry (PyGC/MS) and determined that the amounts of isoprene and limonene released from polyprenols and polyisoprenes were dependent upon their molecular weights. Based on these results, we developed a relative quantification method for polyisoprene in leaves by direct analysis of 1 mg of leaves using PyGC/MS. This novel quantification method eliminated extraction steps and can be used in the measurement of polyisoprene contents in Eucommia ulmoides and Hevea brasiliensis.
UR - http://www.scopus.com/inward/record.url?scp=75649103599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=75649103599&partnerID=8YFLogxK
U2 - 10.1271/bbb.80882
DO - 10.1271/bbb.80882
M3 - Article
C2 - 20057156
AN - SCOPUS:75649103599
SN - 0916-8451
VL - 74
SP - 13
EP - 17
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 1
ER -