TY - GEN
T1 - High-speed micro-RNA isolation from DNA fragments by nanopiller array chip
AU - Wu, Qiong
AU - Yasui, Takao
AU - Rahong, Sakon
AU - Yanagida, Takeshi
AU - Kanai, Masaki
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Nagashima, Kazuki
AU - Kawai, Tomoji
AU - Baba, Yoshinobu
PY - 2013
Y1 - 2013
N2 - A novel nanopillar chip, which combines pillar structures (nanopillars) and dammed structures (nanoslits) at the nanometer scale inside a microchannel, was fabricated and applied to micro-RNA isolation from a mixture of nucleic acids. Electrophoretic behaviors of micro-RNA and DNA fragments in the nanopillar chip were carefully investigated and the isolation condition was optimized for the mixture of 10-kbp, lambda (48.5-kbp) and T4 DNA (165.5-kbp).
AB - A novel nanopillar chip, which combines pillar structures (nanopillars) and dammed structures (nanoslits) at the nanometer scale inside a microchannel, was fabricated and applied to micro-RNA isolation from a mixture of nucleic acids. Electrophoretic behaviors of micro-RNA and DNA fragments in the nanopillar chip were carefully investigated and the isolation condition was optimized for the mixture of 10-kbp, lambda (48.5-kbp) and T4 DNA (165.5-kbp).
UR - http://www.scopus.com/inward/record.url?scp=84907374049&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907374049&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907374049
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 1206
EP - 1208
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -