TY - JOUR
T1 - High cell density cultivation of anchorage-dependent cells using a novel macroporous cellulosic support
AU - Ogawa, Tatsuya
AU - Kamihira, Masamichi
AU - Terashima, Shuji
AU - Yasuda, Kimiaki
AU - Iijima, Shinji
AU - Kobayashi, Takeshi
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid (No. 03650784) for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1992
Y1 - 1992
N2 - We have developed a novel cell support made of macroporous cellulose fibers (SIC) for the culture of anchorage-dependent animal cells. We examined several anchorage-dependent cell lines and found that all of them attached to the support and grew, although the efficiency of cell adsorption to the support differed with cell line. Among the cell lines tested, simian virus 40-transformed human endothelial cell line #3 was immobilized on the support and cultured in a fluidized-bed bioreactor at a high cell density. By direct introduction of air and optimization of the medium feed rate, the cells could grow to 2 × 107 cells per cm3-SIC and be maintained over 80 d.
AB - We have developed a novel cell support made of macroporous cellulose fibers (SIC) for the culture of anchorage-dependent animal cells. We examined several anchorage-dependent cell lines and found that all of them attached to the support and grew, although the efficiency of cell adsorption to the support differed with cell line. Among the cell lines tested, simian virus 40-transformed human endothelial cell line #3 was immobilized on the support and cultured in a fluidized-bed bioreactor at a high cell density. By direct introduction of air and optimization of the medium feed rate, the cells could grow to 2 × 107 cells per cm3-SIC and be maintained over 80 d.
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U2 - 10.1016/0922-338X(92)90263-T
DO - 10.1016/0922-338X(92)90263-T
M3 - Article
AN - SCOPUS:0026704931
SN - 0922-338X
VL - 74
SP - 27
EP - 31
JO - Journal of Fermentation and Bioengineering
JF - Journal of Fermentation and Bioengineering
IS - 1
ER -