High-accuracy analysis system for the redox status of coenzyme Q 10 by online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry

Atsuki Matsubara; Kazuo Harada; Kazumasa Hirata; Eiichiro Fukusaki; Takeshi Bamba

doi:10.1016/j.chroma.2012.05.009

High-accuracy analysis system for the redox status of coenzyme Q ₁₀ by online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry

Atsuki Matsubara, Kazuo Harada, Kazumasa Hirata, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The accurate profiling of oxidative stress markers is often disturbed by oxidation occurred during sample preparation. In this study, an online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry system was constructed utilizing supercritical fluid as an efficient solvent for extraction. A higher ratio of the reduced form of coenzyme Q ₁₀ (CoQ ₁₀) was detected by online extraction, rather than by offline extraction using a conventional organic solvent. The results suggest that the profile of easily oxidized compounds can be acquired with high accuracy using this system because the oxidation of the target compounds during sample preparation can be avoided when using the online extraction.

Original language	English
Pages (from-to)	76-79
Number of pages	4
Journal	Journal of Chromatography A
Volume	1250
DOIs	https://doi.org/10.1016/j.chroma.2012.05.009
Publication status	Published - Aug 10 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2012.05.009

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abstract = "The accurate profiling of oxidative stress markers is often disturbed by oxidation occurred during sample preparation. In this study, an online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry system was constructed utilizing supercritical fluid as an efficient solvent for extraction. A higher ratio of the reduced form of coenzyme Q 10 (CoQ 10) was detected by online extraction, rather than by offline extraction using a conventional organic solvent. The results suggest that the profile of easily oxidized compounds can be acquired with high accuracy using this system because the oxidation of the target compounds during sample preparation can be avoided when using the online extraction.",

author = "Atsuki Matsubara and Kazuo Harada and Kazumasa Hirata and Eiichiro Fukusaki and Takeshi Bamba",

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AU - Matsubara, Atsuki

AU - Harada, Kazuo

AU - Hirata, Kazumasa

AU - Fukusaki, Eiichiro

AU - Bamba, Takeshi

PY - 2012/8/10

Y1 - 2012/8/10

N2 - The accurate profiling of oxidative stress markers is often disturbed by oxidation occurred during sample preparation. In this study, an online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry system was constructed utilizing supercritical fluid as an efficient solvent for extraction. A higher ratio of the reduced form of coenzyme Q 10 (CoQ 10) was detected by online extraction, rather than by offline extraction using a conventional organic solvent. The results suggest that the profile of easily oxidized compounds can be acquired with high accuracy using this system because the oxidation of the target compounds during sample preparation can be avoided when using the online extraction.

AB - The accurate profiling of oxidative stress markers is often disturbed by oxidation occurred during sample preparation. In this study, an online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry system was constructed utilizing supercritical fluid as an efficient solvent for extraction. A higher ratio of the reduced form of coenzyme Q 10 (CoQ 10) was detected by online extraction, rather than by offline extraction using a conventional organic solvent. The results suggest that the profile of easily oxidized compounds can be acquired with high accuracy using this system because the oxidation of the target compounds during sample preparation can be avoided when using the online extraction.

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High-accuracy analysis system for the redox status of coenzyme Q ₁₀ by online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry

Abstract

All Science Journal Classification (ASJC) codes

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