TY - JOUR
T1 - Heterogeneous reorganization of actin filaments in living endothelial cellin response to shear stress
AU - Sera, Toshihiro
AU - Terada, Marie
AU - Kudo, Susumu
N1 - Publisher Copyright:
© Japanese Society of Biorheology 2020.
PY - 2020
Y1 - 2020
N2 - In this study, we investigated the spatial and temporal reorganization of actin filaments in living endothelial cells in response to shear stress by transfecting a fluorescent protein, Dronpa-Green-labeled actin, which was photoactivated microscopically by UV irradiation, and evaluating the time constants of fluorescence decay after photoactivation. The time constant in the upstream region decreased gradually after 30 min of shear stress and then increased. Particularly, the time constant in the downstream region tended to be higher than in the upstream region, suggesting that actin polymerization was more activated in the downstream region. Our results demonstrated the spatial and temporal heterogeneity of actin reorganization due to shear stress.
AB - In this study, we investigated the spatial and temporal reorganization of actin filaments in living endothelial cells in response to shear stress by transfecting a fluorescent protein, Dronpa-Green-labeled actin, which was photoactivated microscopically by UV irradiation, and evaluating the time constants of fluorescence decay after photoactivation. The time constant in the upstream region decreased gradually after 30 min of shear stress and then increased. Particularly, the time constant in the downstream region tended to be higher than in the upstream region, suggesting that actin polymerization was more activated in the downstream region. Our results demonstrated the spatial and temporal heterogeneity of actin reorganization due to shear stress.
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U2 - 10.17106/jbr.34.18
DO - 10.17106/jbr.34.18
M3 - Article
AN - SCOPUS:85087105952
SN - 1867-0466
VL - 34
SP - 18
EP - 24
JO - Journal of Biorheology
JF - Journal of Biorheology
IS - 1
ER -