Herbal drug ninjin'yoeito accelerates myelopoiesis but not erythropoiesis in vitro

Tomoko Inoue, Kasem Kulkeaw, Kanitta Muennu, Yuka Tanaka, Yoichi Nakanishi, Daisuke Sugiyama

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Some Kampo medicines that are herbal and traditional in Japan have had beneficial effects when given to patients with anemia. However, molecular mechanisms underlying their effects are unclear. To address this question, four Kampo medicines used to treat anemia-ninjin'yoeito (NYT), shimotsuto (SMT), juzentaihoto (JTT), and daibofuto (DBT)-were tested separately using in vitro cultures of mouse bone marrow mononuclear cells. Among them, NYT was most effective in stimulating cell proliferation and up-regulating Myc expression. Flow cytometry analysis indicated that, among hematopoietic components of those cultures, myeloid cells expressing CD45/Mac-1/Gr-1/F4/80 increased in number, but Ter119/CD71 erythroid cells did not. Accordingly, real-time PCR analysis showed up-regulation of the myeloid gene Pu.1, whereas the erythroid genes Gata1 and Klf1 were down-regulated. Overall, these findings provide molecular evidence that NYT accelerates myelopoiesis but not erythropoiesis in vitro.

Original languageEnglish
Pages (from-to)432-440
Number of pages9
JournalGenes to Cells
Issue number5
Publication statusPublished - May 2014

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology


Dive into the research topics of 'Herbal drug ninjin'yoeito accelerates myelopoiesis but not erythropoiesis in vitro'. Together they form a unique fingerprint.

Cite this