Heparin-conjugated gelatin as a growth factor immobilization scaffold

Shintaro Nakamura, Takafumi Kubo, Hiroyuki Ijima

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Tissue engineering requires growth factors, cells and a scaffold to permit effective tissue regeneration. This study aimed to develop a scaffold with a focus on immobilizing growth factors within gelatin. We focused on the extracellular matrix and developed a heparin-conjugated gelatin (Hep-gela). Conjugation was confirmed using the alcian blue assay and X-ray diffraction patterns. The mechanical strength and stability of the Hep-gela gel in protease solution were improved compared with collagen gel. Hep-gela was able to immobilize vascular endothelial growth factor (VEGF) even in the presence of albumin, with an efficiency of 54.2%. Immobilized VEGF promoted proliferation of human umbilical vein endothelial cells. Hep-gela-immobilized VEGF maintained its native biological activity. In summary, Hep-gela has the potential to become an effective material in the field of regenerative medicine.

Original languageEnglish
Pages (from-to)562-567
Number of pages6
JournalJournal of Bioscience and Bioengineering
Issue number5
Publication statusPublished - May 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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