Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system

Kounosuke Hayashi; Jae Man Lee; Yusuke Tomozoe; Takahiro Kusakabe; Noriho Kamiya

doi:10.1016/j.jbiosc.2015.02.013

Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system

Kounosuke Hayashi, Jae Man Lee, Yusuke Tomozoe, Takahiro Kusakabe, Noriho Kamiya

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Recombinant peroxidase from Arthromyces ramosus, fused with domains of antibody-binding proteins, was successfully obtained by a silkworm larvae expression system. The catalytic activity of the fusion peroxidase was increased 6-fold with the injection of 5-aminolevulinic acid into silkworm larvae as a heme precursor.

Original language	English
Pages (from-to)	384-386
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	120
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiosc.2015.02.013
Publication status	Published - Oct 1 2015

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2015.02.013

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title = "Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system",

abstract = "Recombinant peroxidase from Arthromyces ramosus, fused with domains of antibody-binding proteins, was successfully obtained by a silkworm larvae expression system. The catalytic activity of the fusion peroxidase was increased 6-fold with the injection of 5-aminolevulinic acid into silkworm larvae as a heme precursor.",

author = "Kounosuke Hayashi and Lee, {Jae Man} and Yusuke Tomozoe and Takahiro Kusakabe and Noriho Kamiya",

note = "Funding Information: This work was supported by Grant-in-aid for Scientific Research (B) (Grant Number 25289297 ). Publisher Copyright: {\textcopyright} 2015 The Society for Biotechnology, Japan.",

year = "2015",

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doi = "10.1016/j.jbiosc.2015.02.013",

language = "English",

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journal = "Journal of Bioscience and Bioengineering",

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publisher = "The Society for Biotechnology, Japan",

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T1 - Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system

AU - Hayashi, Kounosuke

AU - Lee, Jae Man

AU - Tomozoe, Yusuke

AU - Kusakabe, Takahiro

AU - Kamiya, Noriho

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Recombinant peroxidase from Arthromyces ramosus, fused with domains of antibody-binding proteins, was successfully obtained by a silkworm larvae expression system. The catalytic activity of the fusion peroxidase was increased 6-fold with the injection of 5-aminolevulinic acid into silkworm larvae as a heme precursor.

AB - Recombinant peroxidase from Arthromyces ramosus, fused with domains of antibody-binding proteins, was successfully obtained by a silkworm larvae expression system. The catalytic activity of the fusion peroxidase was increased 6-fold with the injection of 5-aminolevulinic acid into silkworm larvae as a heme precursor.

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DO - 10.1016/j.jbiosc.2015.02.013

M3 - Article

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AN - SCOPUS:84940897439

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JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 4

ER -

Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system

Abstract

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