TY - JOUR
T1 - GSK3beta inhibitor-induced dental mesenchymal stem cells regulate ameloblast differentiation
AU - Yamada, Aya
AU - Yoshizaki, Keigo
AU - Saito, Kan
AU - Ishikawa, Masaki
AU - Chiba, Yuta
AU - Hoshikawa, Seira
AU - Chiba, Mitsuki
AU - Hino, Ryoko
AU - Maruya, Yuriko
AU - Sato, Hiroshi
AU - Masuda, Keiji
AU - Yamaza, Haruyoshi
AU - Nakamura, Takashi
AU - Iwamoto, Tsutomu
AU - Fukumoto, Satoshi
N1 - Funding Information:
We thank Yoshihiko Yamada (NIH/NIDCR) for consulting on this study. We thank Editage (www.editage.com) for the English language editing. This study was supported by a Grant-in-Aid from the Japan Society for the Promotion of Science (JSPS) KAKENHI (JP24390460 and JP22H03296 to AY, JP17H01606, JP20K20612 and JP22H00488 to SF, and JP21H03150 to KY).
Funding Information:
We thank Yoshihiko Yamada (NIH/NIDCR) for consulting on this study. We thank Editage ( www.editage.com ) for the English language editing. This study was supported by a Grant-in-Aid from the Japan Society for the Promotion of Science ( JSPS ) KAKENHI (JP24390460 and JP22H03296 to AY, JP17H01606, JP20K20612 and JP22H00488 to SF , and JP21H03150 to KY).
Publisher Copyright:
© 2022 Japanese Association for Oral Biology
PY - 2022/12
Y1 - 2022/12
N2 - Objectives: Epithelial-mesenchymal interactions are extremely important in tooth development and essential for ameloblast differentiation, especially during tooth formation. We aimed to identify the type of mesenchymal cells important in ameloblast differentiation. Methods: We used two types of cell culture systems with chambers and found that a subset of debtal mesenchimal cells is important for the differentiatiuon of dental spithelial cells into ameloblasts. Further, we induced dental pulp stem cell-like cells from dental pulp stem cells using the small molecule compound BIO ( a GSK-3 inhibitor IX) to clarify the mechanism involved in ameloblast differentiation induced by dental pulp stem cells. Results: The BIO-induced dental pulp cells promoted the expression of mesenchymal stem cell markers Oct3/4 and Bcrp1. Furthermore, we used artificial dental pulp stem cells induced by BIO to identify the molecules expressed in dental pulp stem cells required for ameloblast differentiation. Panx3 expression was induced in the dental pulp stem cell through interaction with the dental epithelial cells. In addition, ATP release from cells increased in Panx3-expressing cells. We also confirmed that ATP stimulation is accepted in dental epithelial cells. Conclusions: These results showed that the Panx3 expressed in dental pulp stem cells is important for ameloblast differentiation and that ATP release by Panx3 may play a role in epithelial–mesenchymal interaction.
AB - Objectives: Epithelial-mesenchymal interactions are extremely important in tooth development and essential for ameloblast differentiation, especially during tooth formation. We aimed to identify the type of mesenchymal cells important in ameloblast differentiation. Methods: We used two types of cell culture systems with chambers and found that a subset of debtal mesenchimal cells is important for the differentiatiuon of dental spithelial cells into ameloblasts. Further, we induced dental pulp stem cell-like cells from dental pulp stem cells using the small molecule compound BIO ( a GSK-3 inhibitor IX) to clarify the mechanism involved in ameloblast differentiation induced by dental pulp stem cells. Results: The BIO-induced dental pulp cells promoted the expression of mesenchymal stem cell markers Oct3/4 and Bcrp1. Furthermore, we used artificial dental pulp stem cells induced by BIO to identify the molecules expressed in dental pulp stem cells required for ameloblast differentiation. Panx3 expression was induced in the dental pulp stem cell through interaction with the dental epithelial cells. In addition, ATP release from cells increased in Panx3-expressing cells. We also confirmed that ATP stimulation is accepted in dental epithelial cells. Conclusions: These results showed that the Panx3 expressed in dental pulp stem cells is important for ameloblast differentiation and that ATP release by Panx3 may play a role in epithelial–mesenchymal interaction.
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U2 - 10.1016/j.job.2022.10.002
DO - 10.1016/j.job.2022.10.002
M3 - Article
C2 - 36270608
AN - SCOPUS:85141327250
SN - 1349-0079
VL - 64
SP - 400
EP - 409
JO - journal of oral biosciences
JF - journal of oral biosciences
IS - 4
ER -