TY - JOUR
T1 - Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants
AU - Correa, D. R.N.
AU - Rocha, L. A.
AU - Ribeiro, A. R.
AU - Gemini-Piperni, S.
AU - Archanjo, B. S.
AU - Achete, C. A.
AU - Werckmann, J.
AU - Afonso, C. R.M.
AU - Shimabukuro, M.
AU - Doi, H.
AU - Tsutsumi, Y.
AU - Hanawa, T.
N1 - Funding Information:
The research was supported by Brazilian agencies funding CNPq (grant # 207417/2015-6 ), CAPES (grant # 99999.008666/2014-08 ) and FAPESP (grant # 00851-6/2015 ), and also by Japan Agency for Medical Research and Development (AMED) , International Collaborative Research Program: Strategic International Research Cooperative Program (SICP) , No. 16jm0310021h0004 and The Light Metal Educational Foundation, Inc. The authors are grateful to Gabriela Castro for the support in the biological assays. A. R. Ribeiro acknowledges the Jovem Cientista do Nosso Estado (2017) and PROPESQ-UNIGRANRIO/FUNADESP fellowships.
Publisher Copyright:
© 2017
PY - 2018/6/25
Y1 - 2018/6/25
N2 - In this study, a micro-arc oxidation treatment was applied to Ti-15Zr-xMo (x = 0, 5, 10 and 15 wt%) alloys to produce porous oxide layers enriched with bioactive ions (calcium and phosphorus) for use as osseointegrative implants. Biocompatibility studies, namely metabolic activity, mineralization and differentiation studies were conducted with human osteoblastic cell line SAOS-2. A typical porous coating was obtained in all samples, with similar morphologies and thicknesses, which were found to be dependent on the maximum applied voltage. Calcium and phosphorus ions were incorporated into the films, as indicated by EDX analysis. Chemical analyses indicated that the films were composed preferentially of Ti and Zr oxides. XRD patterns revealed mostly substrate Ti phases. However, cross-sectional TEM imaging and automated phase and orientation mapping showed distinct amorphous and nanocrystalline regions within the films, with a higher fraction of Ca atoms incorporated in the outer layer. After immersion in Hanks’ Balanced Salt Solution (HBSS) for seven days, small amounts of calcium phosphate precipitates were observed at the surface of all samples which were confirmed by ICP-AES measurements, indicating that the MAO treatment possibly introduced a considerable bioactive response in the samples. Biological results indicate that Ti-15Zr-15Mo MAO-treated surfaces are biocompatible and induce a higher osteoblasts viability and mineralization. The combination of porous structure and bioactive composition of the oxide layers can be suitable for use as advanced biomedical implants with osseointegration ability.
AB - In this study, a micro-arc oxidation treatment was applied to Ti-15Zr-xMo (x = 0, 5, 10 and 15 wt%) alloys to produce porous oxide layers enriched with bioactive ions (calcium and phosphorus) for use as osseointegrative implants. Biocompatibility studies, namely metabolic activity, mineralization and differentiation studies were conducted with human osteoblastic cell line SAOS-2. A typical porous coating was obtained in all samples, with similar morphologies and thicknesses, which were found to be dependent on the maximum applied voltage. Calcium and phosphorus ions were incorporated into the films, as indicated by EDX analysis. Chemical analyses indicated that the films were composed preferentially of Ti and Zr oxides. XRD patterns revealed mostly substrate Ti phases. However, cross-sectional TEM imaging and automated phase and orientation mapping showed distinct amorphous and nanocrystalline regions within the films, with a higher fraction of Ca atoms incorporated in the outer layer. After immersion in Hanks’ Balanced Salt Solution (HBSS) for seven days, small amounts of calcium phosphate precipitates were observed at the surface of all samples which were confirmed by ICP-AES measurements, indicating that the MAO treatment possibly introduced a considerable bioactive response in the samples. Biological results indicate that Ti-15Zr-15Mo MAO-treated surfaces are biocompatible and induce a higher osteoblasts viability and mineralization. The combination of porous structure and bioactive composition of the oxide layers can be suitable for use as advanced biomedical implants with osseointegration ability.
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U2 - 10.1016/j.surfcoat.2018.02.099
DO - 10.1016/j.surfcoat.2018.02.099
M3 - Article
AN - SCOPUS:85044137384
SN - 0257-8972
VL - 344
SP - 373
EP - 382
JO - Surface and Coatings Technology
JF - Surface and Coatings Technology
ER -