TY - JOUR
T1 - Gonadotropin-inhibiting hormone stimulates feeding behavior in chicks
AU - Tachibana, Tetsuya
AU - Sato, Momoka
AU - Takahashi, Hirokazu
AU - Ukena, Kazuyoshi
AU - Tsutsui, Kazuyoshi
AU - Furuse, Mitsuhiro
N1 - Funding Information:
The authors are grateful to Dr. D.M. Denbow, Virginia Polytechnic Institute and State University, USA, for his critical reading of the manuscript. This work was supported by Grant-in-Aid for Young Scientists (No. 15780187), Grant-in-Aid for Scientific Research from Japan Society for the Promotion of Science (No. 16380191 and 17208023) and Inamori Foundation.
PY - 2005/7/19
Y1 - 2005/7/19
N2 - Neuropeptides containing a C-terminal Arg-Phe-NH2 motif (RFamide peptides) are suggested to be involved in the control of feeding behavior in both invertebrates and vertebrates. Gonadotropin-inhibitory hormone (GnIH) is the first identified avian RFamide peptide that inhibits gonadotropin release from the pituitary. The GnIH precursor encodes one GnIH and its related peptides (GnIH-RP-1 and -RP-2) that shared the same C-terminal motif, Leu-Pro-Xaa-Arg-Phe-NH2 (Xaa = Leu or Gln) (LPXRFamide). GnIH neurons are localized in the paraventricular nucleus, with their fibers visible in multiple brain locations including the median eminence and brainstem. In this study, we therefore investigated the action of GnIH and its related peptides on feeding behavior. Intracerebroventricular (ICV) injection of GnIH, GnIH-RP-1 and GnIH-RP-2 significantly stimulated food intake in chicks. The chicken pentapeptide LPLRFamide, a degraded C-terminus of GnIH and GnIH-RP-1, did not stimulate feeding thereby demonstrating the importance of the N-terminus of GnIH and its related peptides for the orexigenic effect. Anti-GnIH antiserum suppressed appetite induced by fasting, but did not modify feeding under ad libitum conditions. The present study suggests that GnIH and its related peptides act as endogenous orexigenic factors in the brain of chicks.
AB - Neuropeptides containing a C-terminal Arg-Phe-NH2 motif (RFamide peptides) are suggested to be involved in the control of feeding behavior in both invertebrates and vertebrates. Gonadotropin-inhibitory hormone (GnIH) is the first identified avian RFamide peptide that inhibits gonadotropin release from the pituitary. The GnIH precursor encodes one GnIH and its related peptides (GnIH-RP-1 and -RP-2) that shared the same C-terminal motif, Leu-Pro-Xaa-Arg-Phe-NH2 (Xaa = Leu or Gln) (LPXRFamide). GnIH neurons are localized in the paraventricular nucleus, with their fibers visible in multiple brain locations including the median eminence and brainstem. In this study, we therefore investigated the action of GnIH and its related peptides on feeding behavior. Intracerebroventricular (ICV) injection of GnIH, GnIH-RP-1 and GnIH-RP-2 significantly stimulated food intake in chicks. The chicken pentapeptide LPLRFamide, a degraded C-terminus of GnIH and GnIH-RP-1, did not stimulate feeding thereby demonstrating the importance of the N-terminus of GnIH and its related peptides for the orexigenic effect. Anti-GnIH antiserum suppressed appetite induced by fasting, but did not modify feeding under ad libitum conditions. The present study suggests that GnIH and its related peptides act as endogenous orexigenic factors in the brain of chicks.
UR - http://www.scopus.com/inward/record.url?scp=21544441490&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21544441490&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2005.05.035
DO - 10.1016/j.brainres.2005.05.035
M3 - Article
C2 - 15979587
AN - SCOPUS:21544441490
SN - 0006-8993
VL - 1050
SP - 94
EP - 100
JO - Brain Research
JF - Brain Research
IS - 1-2
ER -