TY - JOUR
T1 - Germ cell-intrinsic effects of sex chromosomes on early oocyte differentiation in mice
AU - Hamada, Norio
AU - Hamazaki, Nobuhiko
AU - Shimamoto, So
AU - Hikabe, Orie
AU - Nagamatsu, Go
AU - Takada, Yuki
AU - Kato, Kiyoko
AU - Hayashi, Katsuhiko
N1 - Funding Information:
This study was supported by KAKENHI Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (nos. 25290033, 25114006, 17H01395, 18H05544 and 18H05545) to K.H. Also, by KAKENHI Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (18K06261) to G.N. Management Expenses Grants of Kyushu University to K.H. and K.K., The Open Philanthropy Project to K.H., The Uehara Memorial Foundation to K.H., The Takeda Science Foundation to K.H. Also, by a Hayashi Grant-in-Aid for Basic Medical Research, Kyushu University to K.H., a JSPS Research Fellowship PD to N. Hamazaki, a JSPS Research Fellowship DC1 to S. S., and a “FUKUOKA” OBGYN Researcher's Charity Foundation Fund to N.Hamada. None of these sponsors and funders play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
Copyright: © 2020 Hamada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2020/3
Y1 - 2020/3
N2 - A set of sex chromosomes is required for gametogenesis in both males and females, as represented by sex chromosome disorders causing agametic phenotypes. Although studies using model animals have investigated the functional requirement of sex chromosomes, involvement of these chromosomes in gametogenesis remains elusive. Here, we elicit a germ cell-intrinsic effect of sex chromosomes on oogenesis, using a novel culture system in which oocytes were induced from embryonic stem cells (ESCs) harboring XX, XO or XY. In the culture system, oogenesis using XO and XY ESCs was severely disturbed, with XY ESCs being more strongly affected. The culture system revealed multiple defects in the oogenesis of XO and XY ESCs, such as delayed meiotic entry and progression, and mispairing of the homologous chromosomes. Interestingly, Eif2s3y, a Y-linked gene that promotes proliferation of spermatogonia, had an inhibitory effect on oogenesis. This led us to the concept that male and female gametogenesis appear to be in mutual conflict at an early stage. This study provides a deeper understanding of oogenesis under a sex-reversal condition.
AB - A set of sex chromosomes is required for gametogenesis in both males and females, as represented by sex chromosome disorders causing agametic phenotypes. Although studies using model animals have investigated the functional requirement of sex chromosomes, involvement of these chromosomes in gametogenesis remains elusive. Here, we elicit a germ cell-intrinsic effect of sex chromosomes on oogenesis, using a novel culture system in which oocytes were induced from embryonic stem cells (ESCs) harboring XX, XO or XY. In the culture system, oogenesis using XO and XY ESCs was severely disturbed, with XY ESCs being more strongly affected. The culture system revealed multiple defects in the oogenesis of XO and XY ESCs, such as delayed meiotic entry and progression, and mispairing of the homologous chromosomes. Interestingly, Eif2s3y, a Y-linked gene that promotes proliferation of spermatogonia, had an inhibitory effect on oogenesis. This led us to the concept that male and female gametogenesis appear to be in mutual conflict at an early stage. This study provides a deeper understanding of oogenesis under a sex-reversal condition.
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U2 - 10.1371/journal.pgen.1008676
DO - 10.1371/journal.pgen.1008676
M3 - Article
C2 - 32214314
AN - SCOPUS:85083003082
SN - 1553-7390
VL - 16
JO - PLoS genetics
JF - PLoS genetics
IS - 3
M1 - e1008676
ER -
