TY - JOUR
T1 - Genomic characterization of β-glucuronidase–positive escherichia coli O157:H7 producing Stx2a
AU - Ogura, Yoshitoshi
AU - Seto, Kazuko
AU - Morimoto, Yo
AU - Nakamura, Keiji
AU - Sato, Mitsuhiko P.
AU - Gotoh, Yasuhiro
AU - Itoh, Takehiko
AU - Toyoda, Atsushi
AU - Ohnishi, Makoto
AU - Hayashi, Tetsuya
N1 - Funding Information:
We thank A. Yoshida, Y. Inoue, M. Shimbara, N. Kanemaru, N. Kawano, N. Sakamoto, H. Iguchi, M. Horiguchi, M. Kumagai, and Y. Morita for providing technical assistance. This work was funded by the Japan Society for the Promotion of Science (KAKENHI) under grant nos. 16K15278 and 17H04077 to Y.O. and 16H05190 to T.H. and by the Japan Agency for Medical Research and Development under grant nos. JP17efk0108127h0001 to Y.O. and JP17fk0108308j0003 to T.H.
Funding Information:
This work was funded by the Japan Society for the Promotion of Science (KAKENHI) under grant nos. 16K15278 and 17H04077 to Y.O. and 16H05190 to T.H. and by the Japan Agency for Medical Research and Development under grant nos. JP17efk0108127h0001 to Y.O. and JP17fk0108308j0003 to T.H.
Publisher Copyright:
© 2018, Centers for Disease Control and Prevention (CDC). All rights reserved.
PY - 2018/12
Y1 - 2018/12
N2 - Among Shiga toxin (Stx)–producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase–positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/ stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (β-glucuronidase–negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones.
AB - Among Shiga toxin (Stx)–producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase–positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/ stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (β-glucuronidase–negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones.
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U2 - 10.3201/eid2412.180404
DO - 10.3201/eid2412.180404
M3 - Article
C2 - 30457544
AN - SCOPUS:85056741225
SN - 1080-6040
VL - 24
SP - 2219
EP - 2227
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 12
ER -
