Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori

Li Zhu; Zhiqing Li; Tsuneyuki Tatsuke; Daojun Cheng; Jian Xu; Kaito Yoshimura; Hiroaki Mon; Kazuhiro Iiyama; Jae Man Lee; Qingyou Xia; Takahiro Kusakabe

doi:10.11416/jibs.82.1_019

Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori

Li Zhu, Zhiqing Li, Tsuneyuki Tatsuke, Daojun Cheng, Jian Xu, Kaito Yoshimura, Hiroaki Mon, Kazuhiro Iiyama, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe

Agricultural Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Using a cDNA microarray, we monitored global gene expression profiles in silkworm BmN4-SID1 cells after the knockdown of BmAGO1 and BmAGO2 genes. Interestingly, there was a significant overlap between the target genes up-regulated by BmAgo1 (292 out of 481) and BmAgo2 (292 out of 361). Of these, the Replicase gene of macula-like virus (BmMLV) was highly induced in both of BmAgo1- and BmAgo2-depleted cells. RT-PCR analysis confirmed that the knockdown of silkworm RNAi pathway-related genes increased the expression of the Replicase gene. These data strongly suggested that the RNAi pathway negatively regulated BmMLV proliferation in silkworm BmN4 cells.

Original language	English
Pages (from-to)	19-23
Number of pages	5
Journal	Journal of Insect Biotechnology and Sericology
Volume	82
Issue number	1
DOIs	https://doi.org/10.11416/jibs.82.1_019
Publication status	Published - Aug 5 2013

All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology
Business, Management and Accounting(all)
Agricultural and Biological Sciences(all)
Insect Science
Industrial and Manufacturing Engineering

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10.11416/jibs.82.1_019

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Zhu, L., Li, Z., Tatsuke, T., Cheng, D., Xu, J., Yoshimura, K., Mon, H., Iiyama, K., Lee, J. M., Xia, Q., & Kusakabe, T. (2013). Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori. Journal of Insect Biotechnology and Sericology, 82(1), 19-23. https://doi.org/10.11416/jibs.82.1_019

Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori. / Zhu, Li; Li, Zhiqing; Tatsuke, Tsuneyuki et al.
In: Journal of Insect Biotechnology and Sericology, Vol. 82, No. 1, 05.08.2013, p. 19-23.

Research output: Contribution to journal › Article › peer-review

Zhu, L, Li, Z, Tatsuke, T, Cheng, D, Xu, J, Yoshimura, K, Mon, H , Iiyama, K , Lee, JM, Xia, Q & Kusakabe, T 2013, 'Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori', Journal of Insect Biotechnology and Sericology, vol. 82, no. 1, pp. 19-23. https://doi.org/10.11416/jibs.82.1_019

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abstract = "Using a cDNA microarray, we monitored global gene expression profiles in silkworm BmN4-SID1 cells after the knockdown of BmAGO1 and BmAGO2 genes. Interestingly, there was a significant overlap between the target genes up-regulated by BmAgo1 (292 out of 481) and BmAgo2 (292 out of 361). Of these, the Replicase gene of macula-like virus (BmMLV) was highly induced in both of BmAgo1- and BmAgo2-depleted cells. RT-PCR analysis confirmed that the knockdown of silkworm RNAi pathway-related genes increased the expression of the Replicase gene. These data strongly suggested that the RNAi pathway negatively regulated BmMLV proliferation in silkworm BmN4 cells.",

author = "Li Zhu and Zhiqing Li and Tsuneyuki Tatsuke and Daojun Cheng and Jian Xu and Kaito Yoshimura and Hiroaki Mon and Kazuhiro Iiyama and Lee, {Jae Man} and Qingyou Xia and Takahiro Kusakabe",

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AU - Zhu, Li

AU - Li, Zhiqing

AU - Tatsuke, Tsuneyuki

AU - Cheng, Daojun

AU - Xu, Jian

AU - Yoshimura, Kaito

AU - Mon, Hiroaki

AU - Iiyama, Kazuhiro

AU - Lee, Jae Man

AU - Xia, Qingyou

AU - Kusakabe, Takahiro

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AB - Using a cDNA microarray, we monitored global gene expression profiles in silkworm BmN4-SID1 cells after the knockdown of BmAGO1 and BmAGO2 genes. Interestingly, there was a significant overlap between the target genes up-regulated by BmAgo1 (292 out of 481) and BmAgo2 (292 out of 361). Of these, the Replicase gene of macula-like virus (BmMLV) was highly induced in both of BmAgo1- and BmAgo2-depleted cells. RT-PCR analysis confirmed that the knockdown of silkworm RNAi pathway-related genes increased the expression of the Replicase gene. These data strongly suggested that the RNAi pathway negatively regulated BmMLV proliferation in silkworm BmN4 cells.

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Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori

Abstract

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