Genome-wide analysis of chlamydophila pneumoniae gene expression at the late stage of infection

Koshiro Miura, Hidehiro Toh, Hideki Hirakawa, Manabu Sugii, Masayuki Murata, Kenta Nakai, Kosuke Tashiro, Satoru Kuhara, Yoshinao Azuma, Mutsunori Shirai

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Chlamydophila pneumoniae, an obligate intracellular eubacterium, changes its form from a vegetative reticulate body into an infectious elementary body during the late stage of its infection cycle. Comprehension of the molecular events in the morphological change is important to understand the switching mechanism between acute and chronic infection, which is deemed to relate to the pathogenesis of atherosclerosis. Herein, we have attempted to screen genes expressed in the late stage with a genome-wide DNA microarray, resulting in nomination of 17 genes as the late-stage genes. Fourteen of the 17 genes and six other genes predicted as late-stage genes were confirmed to be up-regulated in the late stage with a quantitative reverse transcriptase-polymerase chain reaction. These 20 late-stage genes were classified into two groups by clustering analysis: 'drastically induced' and 'moderately induced' genes. Out of eight drastically induced genes, four contain s28 promoter-like sequences and the other four contain an upstream common sequence. It suggests that besides s28, there are certain up-regulatory mechanisms at the late stage, which may be involved in the chlamydial morphological change and thus pathogenesis.

Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalDNA Research
Issue number2
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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