Genetic characterization of the rice sheath blight pathogen Rhizoctonia solani AG1-IA in North Vietnam by rep-pcr and sequence analysis

M. Matsumoto; H. V. Cuong

doi:10.4454/JPP.V96I2.018

Genetic characterization of the rice sheath blight pathogen Rhizoctonia solani AG1-IA in North Vietnam by rep-pcr and sequence analysis

M. Matsumoto, H. V. Cuong

Institute of Tropical Agriculture

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Representative isolates of Rhizoctonia solani AG1-IA associated with rice sheath blight obtained from North Vietnam were characterized using rep-PCR fingerprinting and ITS-5.8S rDNA sequencing. For rep-PCR assays primers ERIC2 and BOXA1R were used. Sequences of these isolates were aligned with other known R. solani sequences from the GenBank. Rep-PCR analysis revealed that two genotypes (Rep-Vet1 and Rep-Vet2) were present based on the fingerprinting dendrograms having the similarity coefficient score at 75% differentiation. The two clonal populations were scattered and had different geographical localizations in North Vietnam.

Original language	English
Pages (from-to)	377-380
Number of pages	4
Journal	Journal of Plant Pathology
Volume	96
Issue number	2
DOIs	https://doi.org/10.4454/JPP.V96I2.018
Publication status	Published - 2014

All Science Journal Classification (ASJC) codes

Plant Science

Access to Document

10.4454/JPP.V96I2.018

Cite this

@article{6ae6344a345a4800b7f2670f0c8405f2,

title = "Genetic characterization of the rice sheath blight pathogen Rhizoctonia solani AG1-IA in North Vietnam by rep-pcr and sequence analysis",

abstract = "Representative isolates of Rhizoctonia solani AG1-IA associated with rice sheath blight obtained from North Vietnam were characterized using rep-PCR fingerprinting and ITS-5.8S rDNA sequencing. For rep-PCR assays primers ERIC2 and BOXA1R were used. Sequences of these isolates were aligned with other known R. solani sequences from the GenBank. Rep-PCR analysis revealed that two genotypes (Rep-Vet1 and Rep-Vet2) were present based on the fingerprinting dendrograms having the similarity coefficient score at 75% differentiation. The two clonal populations were scattered and had different geographical localizations in North Vietnam.",

author = "M. Matsumoto and Cuong, {H. V.}",

year = "2014",

doi = "10.4454/JPP.V96I2.018",

language = "English",

volume = "96",

pages = "377--380",

journal = "Journal of Plant Pathology",

issn = "1125-4653",

publisher = "Edizioni E.T.S.",

number = "2",

}

TY - JOUR

T1 - Genetic characterization of the rice sheath blight pathogen Rhizoctonia solani AG1-IA in North Vietnam by rep-pcr and sequence analysis

AU - Matsumoto, M.

AU - Cuong, H. V.

PY - 2014

Y1 - 2014

N2 - Representative isolates of Rhizoctonia solani AG1-IA associated with rice sheath blight obtained from North Vietnam were characterized using rep-PCR fingerprinting and ITS-5.8S rDNA sequencing. For rep-PCR assays primers ERIC2 and BOXA1R were used. Sequences of these isolates were aligned with other known R. solani sequences from the GenBank. Rep-PCR analysis revealed that two genotypes (Rep-Vet1 and Rep-Vet2) were present based on the fingerprinting dendrograms having the similarity coefficient score at 75% differentiation. The two clonal populations were scattered and had different geographical localizations in North Vietnam.

AB - Representative isolates of Rhizoctonia solani AG1-IA associated with rice sheath blight obtained from North Vietnam were characterized using rep-PCR fingerprinting and ITS-5.8S rDNA sequencing. For rep-PCR assays primers ERIC2 and BOXA1R were used. Sequences of these isolates were aligned with other known R. solani sequences from the GenBank. Rep-PCR analysis revealed that two genotypes (Rep-Vet1 and Rep-Vet2) were present based on the fingerprinting dendrograms having the similarity coefficient score at 75% differentiation. The two clonal populations were scattered and had different geographical localizations in North Vietnam.

UR - http://www.scopus.com/inward/record.url?scp=84904004039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904004039&partnerID=8YFLogxK

U2 - 10.4454/JPP.V96I2.018

DO - 10.4454/JPP.V96I2.018

M3 - Article

AN - SCOPUS:84904004039

SN - 1125-4653

VL - 96

SP - 377

EP - 380

JO - Journal of Plant Pathology

JF - Journal of Plant Pathology

IS - 2

ER -

Genetic characterization of the rice sheath blight pathogen Rhizoctonia solani AG1-IA in North Vietnam by rep-pcr and sequence analysis

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this