TY - JOUR
T1 - Genetic analysis of cysteine-poor prolamin polypeptides reduced in the endosperm of the rice esp1 mutant
AU - Ushijima, Tomokazu
AU - Matsusaka, Hiroaki
AU - Jikuya, Hiroyuki
AU - Ogawa, Masahiro
AU - Satoh, Hikaru
AU - Kumamaru, Toshihiro
N1 - Funding Information:
We sincerely thank Professor Yoichiro Aso, Kyushu University, for valuable suggestions. This work was supported in part by a grant-in-aid for scientific research from the Japanese Society for the Promotion of Science (21380008); the program for Promotion of Basic Research Activities for Innovative Biosciences from the Bio-oriented Technology Research Advanced Institution (BRAIN).
PY - 2011/8
Y1 - 2011/8
N2 - The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35 are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF) analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in the 44.2. cM region on chromosome 5.
AB - The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35 are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF) analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in the 44.2. cM region on chromosome 5.
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U2 - 10.1016/j.plantsci.2011.04.011
DO - 10.1016/j.plantsci.2011.04.011
M3 - Article
C2 - 21683877
AN - SCOPUS:79959204493
SN - 0168-9452
VL - 181
SP - 125
EP - 131
JO - Plant Science
JF - Plant Science
IS - 2
ER -