TY - GEN
T1 - GENERATION OF TRANSMITOCHONDRIAL CYBRID USING A MICROFLUIDIC DEVICE
AU - Wada, Ken Ichi
AU - Hosokawa, Kazuo
AU - Ito, Yoshihiro
AU - Maeda, Mizuo
AU - Harada, Yui
AU - Yonemitsu, Yoshikazu
N1 - Funding Information:
This work was supported by Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research (KAKENHI) (Grant Numbers 22220009, 26650068 and 16K07207), Center of Innovation Program from Japan Science and Technology Agency, and Single Cell Project from RIKEN.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - We previously developed a microfluidic device which can transfer mitochondria between live single cells by promoting cytoplasmic connection through a microtunnel [1]. In this study, we succeeded to generate transmitochondrial cybrids, or mitochondrial DNA (mtDNA)-replaced cells, using the microfluidic device by performing mitochondrial transfer from a mtDNA-intact cell to a mtDNA-less (ρ0) cell.
AB - We previously developed a microfluidic device which can transfer mitochondria between live single cells by promoting cytoplasmic connection through a microtunnel [1]. In this study, we succeeded to generate transmitochondrial cybrids, or mitochondrial DNA (mtDNA)-replaced cells, using the microfluidic device by performing mitochondrial transfer from a mtDNA-intact cell to a mtDNA-less (ρ0) cell.
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UR - http://www.scopus.com/inward/citedby.url?scp=85136992187&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136992187
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 613
EP - 614
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -