Generation of the Rat Monoclonal Antibody against the Extracellular Domain of Human CD63 by DNA Immunization

Liu Xu; Kan Ichiro Ihara; Saori Yoshimura; Daijiro Konno; Akira Tachibana; Takeshi Nakanishi; Taro Tachibana

doi:10.1089/mab.2020.0007

Generation of the Rat Monoclonal Antibody against the Extracellular Domain of Human CD63 by DNA Immunization

Liu Xu, Kan Ichiro Ihara, Saori Yoshimura, Daijiro Konno, Akira Tachibana, Takeshi Nakanishi, Taro Tachibana

Department of Immunobiology and Neuroscience

Research output: Contribution to journal › Article › peer-review

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Abstract

Human cluster of differentiation 63 (hCD63) is one of the tetraspanin receptors that is abundant on the surface of exosomes. Exosomes are involved in cell-to-cell communication, including from cancer cells to normal cells. It is very important to detect exosomes as a marker for the diagnosis of various diseases. In this study, we report the generation and characterization of a monoclonal antibody (mAb) against the extracellular domain of hCD63 using DNA immunization. This mAb, clone 1C8-2B11, exhibits high performance for use in immunofluorescence and flow cytometry, and it has 10-fold higher affinity than the control antibody that is commercially available. mAb 1C8-2B11 has great potential to be a tool for research and clinical diagnosis.

Original language	English
Pages (from-to)	74-76
Number of pages	3
Journal	Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume	39
Issue number	3
DOIs	https://doi.org/10.1089/mab.2020.0007
Publication status	Published - Jun 2020

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/mab.2020.0007

Cite this

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abstract = "Human cluster of differentiation 63 (hCD63) is one of the tetraspanin receptors that is abundant on the surface of exosomes. Exosomes are involved in cell-to-cell communication, including from cancer cells to normal cells. It is very important to detect exosomes as a marker for the diagnosis of various diseases. In this study, we report the generation and characterization of a monoclonal antibody (mAb) against the extracellular domain of hCD63 using DNA immunization. This mAb, clone 1C8-2B11, exhibits high performance for use in immunofluorescence and flow cytometry, and it has 10-fold higher affinity than the control antibody that is commercially available. mAb 1C8-2B11 has great potential to be a tool for research and clinical diagnosis.",

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AU - Xu, Liu

AU - Ihara, Kan Ichiro

AU - Yoshimura, Saori

AU - Konno, Daijiro

AU - Tachibana, Akira

AU - Nakanishi, Takeshi

AU - Tachibana, Taro

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AB - Human cluster of differentiation 63 (hCD63) is one of the tetraspanin receptors that is abundant on the surface of exosomes. Exosomes are involved in cell-to-cell communication, including from cancer cells to normal cells. It is very important to detect exosomes as a marker for the diagnosis of various diseases. In this study, we report the generation and characterization of a monoclonal antibody (mAb) against the extracellular domain of hCD63 using DNA immunization. This mAb, clone 1C8-2B11, exhibits high performance for use in immunofluorescence and flow cytometry, and it has 10-fold higher affinity than the control antibody that is commercially available. mAb 1C8-2B11 has great potential to be a tool for research and clinical diagnosis.

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Generation of the Rat Monoclonal Antibody against the Extracellular Domain of Human CD63 by DNA Immunization

Abstract

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