TY - JOUR
T1 - Gene product identification and promoter analysis of hig locus of plasmid Rts1
AU - Tian, Qing Bao
AU - Hayashi, Tetsuya
AU - Murata, Takahiro
AU - Terawaki, Yoshiro
N1 - Funding Information:
The authors thank Dr Akira Tabuchi for providing plasmids, Miss Kaori Sato for her technical assistance, and Dr. Makoto Ohnishi and Yong Fang Li for valuable discussions. This work was supported by a Grant-in-aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan and by grants from the Yakult Foundation.
PY - 1996/8/14
Y1 - 1996/8/14
N2 - The hig (host inhibition of growth) genes of plasmid Rts1 belong to the plasmid-encoded proteic killer gene family. Compared with other proteic killer genes described so far, hig is unique in that the toxic part (higB) exists upstream of the antidote gene (higA). Here we describe results of the promoter analysis of hig genes together with identification of the proteic gene products of higA and higB. Two promoters were identified in the hig locus; a stronger one, named Phig, is located upstream of higB and a weaker one, PhigA, is upstream of higA within the higB coding region. The Phig activity was negatively regulated by HigA and this regulation was augmented by HigB, whereas PhigA was not subjected to such a regulation.
AB - The hig (host inhibition of growth) genes of plasmid Rts1 belong to the plasmid-encoded proteic killer gene family. Compared with other proteic killer genes described so far, hig is unique in that the toxic part (higB) exists upstream of the antidote gene (higA). Here we describe results of the promoter analysis of hig genes together with identification of the proteic gene products of higA and higB. Two promoters were identified in the hig locus; a stronger one, named Phig, is located upstream of higB and a weaker one, PhigA, is upstream of higA within the higB coding region. The Phig activity was negatively regulated by HigA and this regulation was augmented by HigB, whereas PhigA was not subjected to such a regulation.
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U2 - 10.1006/bbrc.1996.1229
DO - 10.1006/bbrc.1996.1229
M3 - Article
C2 - 8753818
AN - SCOPUS:0030583276
SN - 0006-291X
VL - 225
SP - 679
EP - 684
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -