Gene expression analysis of CCN protein in bone under mechanical stress

Teruko Takano-Yamamoto, Tomohiro Fukunaga, Nobuo Takeshita

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)


To investigate mechanical-dependent bone remodeling, we had previously applied various types of mechanical loading onto the teeth of rats and mice. In vitro cultured bone cells were then used to elucidate the mechanisms underlying the specifi c phenomenon revealed by in vivo experiments. This review describes the techniques used to upregulate CCN2 expression in bone cells produced by different types of mechanical stress, such as fl uid shear stress and substrate strain in vitro, and compression or tension force in vivo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages26
Publication statusPublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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