TY - JOUR
T1 - Gene encoding a replication initiator protein and replication origin of conjugative plasmid pSA1.1 of Streptomyces cyaneus ATCC 14921
AU - Yokoyama, Eiji
AU - Matsuzaki, Yumi
AU - Doi, Katsumi
AU - Ogata, Seiya
N1 - Funding Information:
This work was supported in part by JSPS Research Fellowships for Young Scientists (No. 7149), a Grant-in-Aid for Scientific Research (B, No. 08456055) from the Ministry of Education, Science, Sports and Culture of Japan and the Waksman Foundation of Japan Inc. We are grateful to M. Ohara for critical comments on the manuscript.
PY - 1998/12/1
Y1 - 1998/12/1
N2 - pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator protein and the replication origin ori of pSA1.1 were determined. The rep and ori are located on separate regions. The Rep protein of pSA1.1 belongs to superfamily I which includes A proteins of phages. Nucleotide sequence of the surrounding putative nicking site of pSA1.1 shows good agreement with those of the pC194 group plasmids and phages. The direction of replication was also determined. Copyright (C) 1998 Federation of European Microbiological Societies.
AB - pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator protein and the replication origin ori of pSA1.1 were determined. The rep and ori are located on separate regions. The Rep protein of pSA1.1 belongs to superfamily I which includes A proteins of phages. Nucleotide sequence of the surrounding putative nicking site of pSA1.1 shows good agreement with those of the pC194 group plasmids and phages. The direction of replication was also determined. Copyright (C) 1998 Federation of European Microbiological Societies.
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U2 - 10.1016/S0378-1097(98)00469-8
DO - 10.1016/S0378-1097(98)00469-8
M3 - Article
C2 - 9851040
AN - SCOPUS:0031763456
SN - 0378-1097
VL - 169
SP - 103
EP - 109
JO - FEMS microbiology letters
JF - FEMS microbiology letters
IS - 1
ER -