Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells

Takashi Kimitsuki; Takashi Nakagawa; Kazutaka Hisashi; Shizuo Komune; Sohtaro Komiyama

doi:10.1016/S0378-5955(96)00134-7

Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells

Takashi Kimitsuki
, Takashi Nakagawa
, Kazutaka Hisashi
, Shizuo Komune
, Sohtaro Komiyama

Surgery

Research output: Contribution to journal › Article › peer-review

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Abstract

We investigated the effects of gadolinium ion (Gd³⁺) on the mechano-electrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd³⁺ blocked the MET channel in a concentration- and voltage-dependent manner. At -50 mV, Gd³⁺ blocked the MET channel, with a Hill coefficient of 1.14 and a dissociation constant (K(D)) of 1.01 x 10^-5 M. Adaptation of the MET current disappeared after the introduction of Gd³⁺, a change that may be due to a decrease in inward going MET currents, specifically the Ca²⁺ component.

Original language	English
Pages (from-to)	75-80
Number of pages	6
Journal	Hearing Research
Volume	101
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-5955(96)00134-7
Publication status	Published - Nov 1996

All Science Journal Classification (ASJC) codes

Sensory Systems

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10.1016/S0378-5955(96)00134-7

Cite this

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title = "Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells",

abstract = "We investigated the effects of gadolinium ion (Gd3+) on the mechano-electrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd3+ blocked the MET channel in a concentration- and voltage-dependent manner. At -50 mV, Gd3+ blocked the MET channel, with a Hill coefficient of 1.14 and a dissociation constant (K(D)) of 1.01 x 10-5 M. Adaptation of the MET current disappeared after the introduction of Gd3+, a change that may be due to a decrease in inward going MET currents, specifically the Ca2+ component.",

author = "Takashi Kimitsuki and Takashi Nakagawa and Kazutaka Hisashi and Shizuo Komune and Sohtaro Komiyama",

note = "Funding Information: The authors appreciate the technical support of Mr. T. Shioya. This work was supported by a Grant-in-Aid (03454406) from the Ministry of Education, Science and Culture of Japan.",

year = "1996",

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doi = "10.1016/S0378-5955(96)00134-7",

language = "English",

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T1 - Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells

AU - Kimitsuki, Takashi

AU - Nakagawa, Takashi

AU - Hisashi, Kazutaka

AU - Komune, Shizuo

AU - Komiyama, Sohtaro

N1 - Funding Information: The authors appreciate the technical support of Mr. T. Shioya. This work was supported by a Grant-in-Aid (03454406) from the Ministry of Education, Science and Culture of Japan.

PY - 1996/11

Y1 - 1996/11

N2 - We investigated the effects of gadolinium ion (Gd3+) on the mechano-electrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd3+ blocked the MET channel in a concentration- and voltage-dependent manner. At -50 mV, Gd3+ blocked the MET channel, with a Hill coefficient of 1.14 and a dissociation constant (K(D)) of 1.01 x 10-5 M. Adaptation of the MET current disappeared after the introduction of Gd3+, a change that may be due to a decrease in inward going MET currents, specifically the Ca2+ component.

AB - We investigated the effects of gadolinium ion (Gd3+) on the mechano-electrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd3+ blocked the MET channel in a concentration- and voltage-dependent manner. At -50 mV, Gd3+ blocked the MET channel, with a Hill coefficient of 1.14 and a dissociation constant (K(D)) of 1.01 x 10-5 M. Adaptation of the MET current disappeared after the introduction of Gd3+, a change that may be due to a decrease in inward going MET currents, specifically the Ca2+ component.

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M3 - Article

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SN - 0378-5955

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JO - Hearing Research

JF - Hearing Research

IS - 1-2

ER -

Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells

Abstract

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