Fundamental study on quasi-real-time detection of airborne bio-particles using discharge plasma

Masudur Rahman, Masakazu Tanino, Mai Hashimoto, Michihiko Nakano, Hachiro Yasuda, Kazunori Takashima, Akira Mizuno

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


A fast detection method for airborne bio-particles (BPs) using electrostatic precipitation (ESP) to enhance trapping and dielectric barrier discharge (DBD) to break the cell wall is demonstrated using Bacillus subtilis as model bacteria. Experimental result shows that 50 times more BPs can be trapped using ESP than without it. Effect of DBD on the B. subtilis under dry and wet conditions was investigated at atmospheric pressure. In wet condition 25 kVp-p DBD successfully broke the B. subtilis cell wall in 40 s. Using electro-blotting the lambda DNA (λDNA) and E. coli genomic DNA were transferred to a nylon membrane, this technique will enhance identification of BPs. These experimental results clearly indicate that discharge plasma can be used to enhance a quasi-real-time detection of airborne BPs.

Original languageEnglish
Pages (from-to)6699-6703
Number of pages5
JournalThin Solid Films
Issue number19
Publication statusPublished - Aug 1 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry


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