Fundamental study on quasi-real-time detection of airborne bio-particles using discharge plasma

Masudur Rahman; Masakazu Tanino; Mai Hashimoto; Michihiko Nakano; Hachiro Yasuda; Kazunori Takashima; Akira Mizuno

doi:10.1016/j.tsf.2007.11.043

Fundamental study on quasi-real-time detection of airborne bio-particles using discharge plasma

Masudur Rahman, Masakazu Tanino, Mai Hashimoto, Michihiko Nakano, Hachiro Yasuda, Kazunori Takashima, Akira Mizuno

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A fast detection method for airborne bio-particles (BPs) using electrostatic precipitation (ESP) to enhance trapping and dielectric barrier discharge (DBD) to break the cell wall is demonstrated using Bacillus subtilis as model bacteria. Experimental result shows that 50 times more BPs can be trapped using ESP than without it. Effect of DBD on the B. subtilis under dry and wet conditions was investigated at atmospheric pressure. In wet condition 25 kV_p-p DBD successfully broke the B. subtilis cell wall in 40 s. Using electro-blotting the lambda DNA (λDNA) and E. coli genomic DNA were transferred to a nylon membrane, this technique will enhance identification of BPs. These experimental results clearly indicate that discharge plasma can be used to enhance a quasi-real-time detection of airborne BPs.

Original language	English
Pages (from-to)	6699-6703
Number of pages	5
Journal	Thin Solid Films
Volume	516
Issue number	19
DOIs	https://doi.org/10.1016/j.tsf.2007.11.043
Publication status	Published - Aug 1 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Surfaces and Interfaces
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1016/j.tsf.2007.11.043

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title = "Fundamental study on quasi-real-time detection of airborne bio-particles using discharge plasma",

abstract = "A fast detection method for airborne bio-particles (BPs) using electrostatic precipitation (ESP) to enhance trapping and dielectric barrier discharge (DBD) to break the cell wall is demonstrated using Bacillus subtilis as model bacteria. Experimental result shows that 50 times more BPs can be trapped using ESP than without it. Effect of DBD on the B. subtilis under dry and wet conditions was investigated at atmospheric pressure. In wet condition 25 kVp-p DBD successfully broke the B. subtilis cell wall in 40 s. Using electro-blotting the lambda DNA (λDNA) and E. coli genomic DNA were transferred to a nylon membrane, this technique will enhance identification of BPs. These experimental results clearly indicate that discharge plasma can be used to enhance a quasi-real-time detection of airborne BPs.",

author = "Masudur Rahman and Masakazu Tanino and Mai Hashimoto and Michihiko Nakano and Hachiro Yasuda and Kazunori Takashima and Akira Mizuno",

note = "Funding Information: The authors are grateful to the Smoking Research Foundation, Japan for the research grant. We are also thankful to Prof. S. Katsura of Gunma University for the valuable discussion, and Prof. Hiraishi and Dr. Futamata of Dept. Ecological Engineering, Toyohashi University of Technology for their assistance on the taking microscopic photographs. ",

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doi = "10.1016/j.tsf.2007.11.043",

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T1 - Fundamental study on quasi-real-time detection of airborne bio-particles using discharge plasma

AU - Rahman, Masudur

AU - Tanino, Masakazu

AU - Hashimoto, Mai

AU - Nakano, Michihiko

AU - Yasuda, Hachiro

AU - Takashima, Kazunori

AU - Mizuno, Akira

N1 - Funding Information: The authors are grateful to the Smoking Research Foundation, Japan for the research grant. We are also thankful to Prof. S. Katsura of Gunma University for the valuable discussion, and Prof. Hiraishi and Dr. Futamata of Dept. Ecological Engineering, Toyohashi University of Technology for their assistance on the taking microscopic photographs.

PY - 2008/8/1

Y1 - 2008/8/1

N2 - A fast detection method for airborne bio-particles (BPs) using electrostatic precipitation (ESP) to enhance trapping and dielectric barrier discharge (DBD) to break the cell wall is demonstrated using Bacillus subtilis as model bacteria. Experimental result shows that 50 times more BPs can be trapped using ESP than without it. Effect of DBD on the B. subtilis under dry and wet conditions was investigated at atmospheric pressure. In wet condition 25 kVp-p DBD successfully broke the B. subtilis cell wall in 40 s. Using electro-blotting the lambda DNA (λDNA) and E. coli genomic DNA were transferred to a nylon membrane, this technique will enhance identification of BPs. These experimental results clearly indicate that discharge plasma can be used to enhance a quasi-real-time detection of airborne BPs.

AB - A fast detection method for airborne bio-particles (BPs) using electrostatic precipitation (ESP) to enhance trapping and dielectric barrier discharge (DBD) to break the cell wall is demonstrated using Bacillus subtilis as model bacteria. Experimental result shows that 50 times more BPs can be trapped using ESP than without it. Effect of DBD on the B. subtilis under dry and wet conditions was investigated at atmospheric pressure. In wet condition 25 kVp-p DBD successfully broke the B. subtilis cell wall in 40 s. Using electro-blotting the lambda DNA (λDNA) and E. coli genomic DNA were transferred to a nylon membrane, this technique will enhance identification of BPs. These experimental results clearly indicate that discharge plasma can be used to enhance a quasi-real-time detection of airborne BPs.

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JO - Thin Solid Films

JF - Thin Solid Films

IS - 19

ER -

Fundamental study on quasi-real-time detection of airborne bio-particles using discharge plasma

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