Functional expression of schizosaccharomyces pombe Vba2p in the vacuolar membrane of Saccharomyces cerevisiae

Pongsanat Pongcharoen; Miyuki Kawano-Kawada; Tomoko Iwaki; Naoko Sugimoto; Takayuki Sekito; Koichi Akiyama; Kaoru Takegawa; Yoshimi Kakinuma

doi:10.1271/bbb.130387

Functional expression of schizosaccharomyces pombe Vba2p in the vacuolar membrane of Saccharomyces cerevisiae

Pongsanat Pongcharoen
, Miyuki Kawano-Kawada
, Tomoko Iwaki
, Naoko Sugimoto
, Takayuki Sekito
, Koichi Akiyama
, Kaoru Takegawa
, Yoshimi Kakinuma

Systems Biology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A vacuolar membrane protein, Vba2p of Schizosaccharomyces pombe, is involved in basic amino acid uptake by intact cells. Here we found evidence that Vba2p mediated ATP-dependent lysine uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae. Vba2p was also responsible for quinidine sensitivity, and the addition of lysine improved cell growth on quinidine-containing media. These findings should be useful for further characterization of Vba2p.

Original language	English
Pages (from-to)	1988-1990
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	77
Issue number	9
DOIs	https://doi.org/10.1271/bbb.130387
Publication status	Published - 2013

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.130387

Cite this

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title = "Functional expression of schizosaccharomyces pombe Vba2p in the vacuolar membrane of Saccharomyces cerevisiae",

abstract = "A vacuolar membrane protein, Vba2p of Schizosaccharomyces pombe, is involved in basic amino acid uptake by intact cells. Here we found evidence that Vba2p mediated ATP-dependent lysine uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae. Vba2p was also responsible for quinidine sensitivity, and the addition of lysine improved cell growth on quinidine-containing media. These findings should be useful for further characterization of Vba2p.",

author = "Pongsanat Pongcharoen and Miyuki Kawano-Kawada and Tomoko Iwaki and Naoko Sugimoto and Takayuki Sekito and Koichi Akiyama and Kaoru Takegawa and Yoshimi Kakinuma",

note = "Funding Information: This work was supported in part by the President{\textquoteright}s Discretionary Budget of Ehime University (to M. K.-K.). P. Pongcharoen is the recipient of a scholarship from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.",

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doi = "10.1271/bbb.130387",

language = "English",

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pages = "1988--1990",

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T1 - Functional expression of schizosaccharomyces pombe Vba2p in the vacuolar membrane of Saccharomyces cerevisiae

AU - Pongcharoen, Pongsanat

AU - Kawano-Kawada, Miyuki

AU - Iwaki, Tomoko

AU - Sugimoto, Naoko

AU - Sekito, Takayuki

AU - Akiyama, Koichi

AU - Takegawa, Kaoru

AU - Kakinuma, Yoshimi

N1 - Funding Information: This work was supported in part by the President’s Discretionary Budget of Ehime University (to M. K.-K.). P. Pongcharoen is the recipient of a scholarship from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

PY - 2013

Y1 - 2013

N2 - A vacuolar membrane protein, Vba2p of Schizosaccharomyces pombe, is involved in basic amino acid uptake by intact cells. Here we found evidence that Vba2p mediated ATP-dependent lysine uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae. Vba2p was also responsible for quinidine sensitivity, and the addition of lysine improved cell growth on quinidine-containing media. These findings should be useful for further characterization of Vba2p.

AB - A vacuolar membrane protein, Vba2p of Schizosaccharomyces pombe, is involved in basic amino acid uptake by intact cells. Here we found evidence that Vba2p mediated ATP-dependent lysine uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae. Vba2p was also responsible for quinidine sensitivity, and the addition of lysine improved cell growth on quinidine-containing media. These findings should be useful for further characterization of Vba2p.

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DO - 10.1271/bbb.130387

M3 - Article

C2 - 24018691

AN - SCOPUS:84884614605

SN - 0916-8451

VL - 77

SP - 1988

EP - 1990

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 9

ER -

Functional expression of schizosaccharomyces pombe Vba2p in the vacuolar membrane of Saccharomyces cerevisiae

Abstract

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