TY - JOUR
T1 - Functional diversification of two bilin reductases for light perception and harvesting in unique cyanobacterium Acaryochloris marina MBIC 11017
AU - Miyake, Keita
AU - Fushimi, Keiji
AU - Kashimoto, Tomonori
AU - Maeda, Kaisei
AU - Ni-Ni-Win,
AU - Kimura, Hiroyuki
AU - Sugishima, Masakazu
AU - Ikeuchi, Masahiko
AU - Narikawa, Rei
N1 - Funding Information:
We thank Dr. Nathan C. Rockwell (UC Davis) and Prof. Nicole Frankenberg‐Dinkel (TU Kaiserslautern) for helpful discussion. The authors would like to thank Enago ( www.enago.jp ) for the English language review. KeM and RN would like to thank fox capture plan and ADAM at for encouragement. This work was supported by JST, CREST (JPMJCR1653 to MI and RN), and JSPS KAKENHI (26702036 to RN).
Publisher Copyright:
© 2020 Federation of European Biochemical Societies
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Bilin pigments play important roles for both light perception and harvesting in cyanobacteria by binding to cyanobacteriochromes (CBCRs) and phycobilisomes (PBS), respectively. Among various cyanobacteria, Acaryochloris marina MBIC 11017 (A. marina 11017) exceptionally uses chlorophyll d as the main photosynthetic pigment absorbing longer wavelength light than the canonical pigment, chlorophyll a, indicating existence of a system to sense longer wavelength light than others. On the other hand, A. marina 11017 has the PBS apparatus to harvest short-wavelength orange light, similar to most cyanobacteria. Thus, A. marina 11017 might sense longer wavelength light and harvest shorter wavelength light by using bilin pigments. Phycocyanobilin (PCB) is the main bilin pigment of both systems. Phycocyanobilin:ferredoxin oxidoreductase (PcyA) catalyzes PCB synthesis from biliverdin via the intermediate 181,182-dihydrobiliverdin (181,182-DHBV), resulting in the stepwise shortening of the absorbing wavelengths. In this study, we found that A. marina 11017 exceptionally encodes two PcyA homologs, AmPcyAc and AmPcyAp. AmPcyAc is encoded on the main chromosome with most photoreceptor genes, whereas AmPcyAp is encoded on a plasmid with PBS-related genes. High accumulation of 181,182-DHBV for extended periods was observed during the reaction catalyzed by AmPcyAc, whereas 181,182-DHBV was transiently accumulated for a short period during the reaction catalyzed by AmPcyAp. CBCRs could sense longer wavelength far-red light through 181,182-DHBV incorporation, whereas PBS could only harvest orange light through PCB incorporation, suggesting functional diversification of PcyA as AmPcyAc and AmPcyAp to provide 181,182-DHBV and PCB to the light perception and harvesting systems, respectively.
AB - Bilin pigments play important roles for both light perception and harvesting in cyanobacteria by binding to cyanobacteriochromes (CBCRs) and phycobilisomes (PBS), respectively. Among various cyanobacteria, Acaryochloris marina MBIC 11017 (A. marina 11017) exceptionally uses chlorophyll d as the main photosynthetic pigment absorbing longer wavelength light than the canonical pigment, chlorophyll a, indicating existence of a system to sense longer wavelength light than others. On the other hand, A. marina 11017 has the PBS apparatus to harvest short-wavelength orange light, similar to most cyanobacteria. Thus, A. marina 11017 might sense longer wavelength light and harvest shorter wavelength light by using bilin pigments. Phycocyanobilin (PCB) is the main bilin pigment of both systems. Phycocyanobilin:ferredoxin oxidoreductase (PcyA) catalyzes PCB synthesis from biliverdin via the intermediate 181,182-dihydrobiliverdin (181,182-DHBV), resulting in the stepwise shortening of the absorbing wavelengths. In this study, we found that A. marina 11017 exceptionally encodes two PcyA homologs, AmPcyAc and AmPcyAp. AmPcyAc is encoded on the main chromosome with most photoreceptor genes, whereas AmPcyAp is encoded on a plasmid with PBS-related genes. High accumulation of 181,182-DHBV for extended periods was observed during the reaction catalyzed by AmPcyAc, whereas 181,182-DHBV was transiently accumulated for a short period during the reaction catalyzed by AmPcyAp. CBCRs could sense longer wavelength far-red light through 181,182-DHBV incorporation, whereas PBS could only harvest orange light through PCB incorporation, suggesting functional diversification of PcyA as AmPcyAc and AmPcyAp to provide 181,182-DHBV and PCB to the light perception and harvesting systems, respectively.
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U2 - 10.1111/febs.15230
DO - 10.1111/febs.15230
M3 - Article
C2 - 31995844
AN - SCOPUS:85079725201
SN - 1742-464X
VL - 287
SP - 4016
EP - 4031
JO - FEBS Journal
JF - FEBS Journal
IS - 18
ER -