TY - JOUR
T1 - Functional Analysis of Human Hepatocytes Isolated From Chimeric Mouse Liver
AU - Harimoto, N.
AU - Nakagawara, H.
AU - Shirabe, K.
AU - Yoshizumi, T.
AU - Itoh, S.
AU - Ikegami, T.
AU - Soejima, Y.
AU - Maehara, Y.
AU - Ishida, Y.
AU - Tateno, C.
AU - Tanaka, Y.
N1 - Funding Information:
Support for this research has been provided by Japan Agency for Medical Research and Development (AMED number: 15fk0310014h0004).
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/12
Y1 - 2018/12
N2 - Chimeric mice with humanized liver were first established by transplanting primary human hepatocytes (PHHs) isolated from a Japanese 27-year-old donor into complementary DNA-urokinase-type plasminogen activator/severe combined immunodeficiency mice. The PHHs from the Japanese donor increased more than 100-fold in the mouse liver, and human hepatocytes purified from the chimeric mouse liver (hcPHs) were successfully transplanted into second-passaged mice. These PHHs and hcPHs can produce human albumin and preserve many liver-specific enzyme genes, which are important for liver function. Interestingly, hepatitis B virus can be infected with these chimeric mice; hepatitis B viral DNA and hepatitis B surface antigen levels were detectable. In conclusion, hcPHs can be an ideal cell source for analysis of human hepatocytes.
AB - Chimeric mice with humanized liver were first established by transplanting primary human hepatocytes (PHHs) isolated from a Japanese 27-year-old donor into complementary DNA-urokinase-type plasminogen activator/severe combined immunodeficiency mice. The PHHs from the Japanese donor increased more than 100-fold in the mouse liver, and human hepatocytes purified from the chimeric mouse liver (hcPHs) were successfully transplanted into second-passaged mice. These PHHs and hcPHs can produce human albumin and preserve many liver-specific enzyme genes, which are important for liver function. Interestingly, hepatitis B virus can be infected with these chimeric mice; hepatitis B viral DNA and hepatitis B surface antigen levels were detectable. In conclusion, hcPHs can be an ideal cell source for analysis of human hepatocytes.
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U2 - 10.1016/j.transproceed.2018.06.035
DO - 10.1016/j.transproceed.2018.06.035
M3 - Article
C2 - 30577278
AN - SCOPUS:85058507199
SN - 0041-1345
VL - 50
SP - 3858
EP - 3862
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 10
ER -
