TY - JOUR
T1 - Functional analysis by inducible RNA interference in Drosophila melanogaster.
AU - Matsushima, Yuichi
AU - Adán, Cristina
AU - Garesse, Rafael
AU - Kaguni, Laurie S.
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2007
Y1 - 2007
N2 - Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become a powerful tool for generating loss-of-function phenotypes. It is used to inactivate genes of interest and represents an elegant approach to genome functional analysis by reverse genetics. In Drosophila, RNA interference has been used in both cell culture and animals. We have adopted this approach to reveal the physiological roles of a number of proteins involved in mitochondrial deoxyribonucleic acid metabolism, and present here experimental schemes to induce the stable expression of double-stranded RNA in Schneider cells and in transgenic Drosophila.
AB - Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become a powerful tool for generating loss-of-function phenotypes. It is used to inactivate genes of interest and represents an elegant approach to genome functional analysis by reverse genetics. In Drosophila, RNA interference has been used in both cell culture and animals. We have adopted this approach to reveal the physiological roles of a number of proteins involved in mitochondrial deoxyribonucleic acid metabolism, and present here experimental schemes to induce the stable expression of double-stranded RNA in Schneider cells and in transgenic Drosophila.
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U2 - 10.1007/978-1-59745-365-3_15
DO - 10.1007/978-1-59745-365-3_15
M3 - Article
C2 - 18314728
AN - SCOPUS:40949086426
SN - 1064-3745
VL - 372
SP - 207
EP - 217
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -