Function of Cyp11a1 in animal models

Meng Chun Hu; Hwei Jan Hsu; Ing Cherng Guo; Bon Chu Chung

doi:10.1016/j.mce.2003.11.024

Function of Cyp11a1 in animal models

Meng Chun Hu
, Hwei Jan Hsu
, Ing Cherng Guo
, Bon Chu Chung

Research output: Contribution to journal › Article › peer-review

73 Citations (Scopus)

Abstract

CYP11A1 encodes the P450scc enzyme that catalyzes the first and rate-limiting step of steroid biosynthesis. It is expressed in the adrenals and gonads under the control of pituitary peptide hormones. Transcription factor SF-1, which binds to the CYP11A1 promoter, plays an important role in the tissue-specific and hormonally regulated expression. Transgenic mouse experiments linking the CYP11A1 promoter to a reporter gene demonstrate the importance of the SF-1-binding site in directing balanced CYP11A1 gene expression. In addition, targeted mutagenesis of Cyp11a1 also reveals the importance of this gene in steroid secretion, gene regulation, and development of genitalia. Zebrafish cyp11a1 is expressed in the gonads and interrenal glands in the adults, similar to mammals. In addition, it is expressed in yolk syncytial layer during early embryogenesis. These two animal models complement each other for the understanding of gene regulatory pathways in human diseases related to steroid imbalance.

Original language	English
Pages (from-to)	95-100
Number of pages	6
Journal	Molecular and Cellular Endocrinology
Volume	215
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mce.2003.11.024
Publication status	Published - Feb 27 2004
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Endocrinology

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10.1016/j.mce.2003.11.024

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title = "Function of Cyp11a1 in animal models",

abstract = "CYP11A1 encodes the P450scc enzyme that catalyzes the first and rate-limiting step of steroid biosynthesis. It is expressed in the adrenals and gonads under the control of pituitary peptide hormones. Transcription factor SF-1, which binds to the CYP11A1 promoter, plays an important role in the tissue-specific and hormonally regulated expression. Transgenic mouse experiments linking the CYP11A1 promoter to a reporter gene demonstrate the importance of the SF-1-binding site in directing balanced CYP11A1 gene expression. In addition, targeted mutagenesis of Cyp11a1 also reveals the importance of this gene in steroid secretion, gene regulation, and development of genitalia. Zebrafish cyp11a1 is expressed in the gonads and interrenal glands in the adults, similar to mammals. In addition, it is expressed in yolk syncytial layer during early embryogenesis. These two animal models complement each other for the understanding of gene regulatory pathways in human diseases related to steroid imbalance.",

author = "Hu, \{Meng Chun\} and Hsu, \{Hwei Jan\} and Guo, \{Ing Cherng\} and Chung, \{Bon Chu\}",

note = "Funding Information: We would like to thank Transgenic Core Facility at Academia Sinica for the generation of transgenic and knockout mouse lines. This work was supported by grants NSC 91-3112-P-001-028-Y (to B.-c. Chung) and NSC90-2320-B002-158 (to I.-C. Guo) from National Science Council, and by Academia Sinica, Republic of China.",

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doi = "10.1016/j.mce.2003.11.024",

language = "English",

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AU - Hu, Meng Chun

AU - Hsu, Hwei Jan

AU - Guo, Ing Cherng

AU - Chung, Bon Chu

N1 - Funding Information: We would like to thank Transgenic Core Facility at Academia Sinica for the generation of transgenic and knockout mouse lines. This work was supported by grants NSC 91-3112-P-001-028-Y (to B.-c. Chung) and NSC90-2320-B002-158 (to I.-C. Guo) from National Science Council, and by Academia Sinica, Republic of China.

PY - 2004/2/27

Y1 - 2004/2/27

N2 - CYP11A1 encodes the P450scc enzyme that catalyzes the first and rate-limiting step of steroid biosynthesis. It is expressed in the adrenals and gonads under the control of pituitary peptide hormones. Transcription factor SF-1, which binds to the CYP11A1 promoter, plays an important role in the tissue-specific and hormonally regulated expression. Transgenic mouse experiments linking the CYP11A1 promoter to a reporter gene demonstrate the importance of the SF-1-binding site in directing balanced CYP11A1 gene expression. In addition, targeted mutagenesis of Cyp11a1 also reveals the importance of this gene in steroid secretion, gene regulation, and development of genitalia. Zebrafish cyp11a1 is expressed in the gonads and interrenal glands in the adults, similar to mammals. In addition, it is expressed in yolk syncytial layer during early embryogenesis. These two animal models complement each other for the understanding of gene regulatory pathways in human diseases related to steroid imbalance.

AB - CYP11A1 encodes the P450scc enzyme that catalyzes the first and rate-limiting step of steroid biosynthesis. It is expressed in the adrenals and gonads under the control of pituitary peptide hormones. Transcription factor SF-1, which binds to the CYP11A1 promoter, plays an important role in the tissue-specific and hormonally regulated expression. Transgenic mouse experiments linking the CYP11A1 promoter to a reporter gene demonstrate the importance of the SF-1-binding site in directing balanced CYP11A1 gene expression. In addition, targeted mutagenesis of Cyp11a1 also reveals the importance of this gene in steroid secretion, gene regulation, and development of genitalia. Zebrafish cyp11a1 is expressed in the gonads and interrenal glands in the adults, similar to mammals. In addition, it is expressed in yolk syncytial layer during early embryogenesis. These two animal models complement each other for the understanding of gene regulatory pathways in human diseases related to steroid imbalance.

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SN - 0303-7207

VL - 215

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EP - 100

JO - Molecular and Cellular Endocrinology

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Function of Cyp11a1 in animal models

Abstract

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