Fractionation of mammalian tissue mRNAs by high-performance gel filtration chromatography

Tadashi Ogishima; Yoshiie Okada; Tsuneo Omura

doi:10.1016/0003-2697(84)90813-3

Fractionation of mammalian tissue mRNAs by high-performance gel filtration chromatography

Tadashi Ogishima, Yoshiie Okada, Tsuneo Omura

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Total RNA was prepared from the free polysomes of bovine adrenal cortex and fractionated by high-performance gel filtration chromatography using a TSK-GEL G4000SW or G5000PW column. The former column gave better separation of 28 and 18 S rRNA than did the latter column when a suitable elution condition was selected. The separation of mRNAs by gel filtration chromatography was examined by in vitro translation of the fractionated RNA samples, and the fractionation of mRNAs roughly according to their sizes was confirmed. Significant enrichment of the mRNA of cytochrome P-450(SCC), whose in vitro-synthesized peptide had a molecular weight of 55,000, was achieved.

Original language	English
Pages (from-to)	309-313
Number of pages	5
Journal	Analytical Biochemistry
Volume	138
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(84)90813-3
Publication status	Published - May 1 1984
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(84)90813-3

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abstract = "Total RNA was prepared from the free polysomes of bovine adrenal cortex and fractionated by high-performance gel filtration chromatography using a TSK-GEL G4000SW or G5000PW column. The former column gave better separation of 28 and 18 S rRNA than did the latter column when a suitable elution condition was selected. The separation of mRNAs by gel filtration chromatography was examined by in vitro translation of the fractionated RNA samples, and the fractionation of mRNAs roughly according to their sizes was confirmed. Significant enrichment of the mRNA of cytochrome P-450(SCC), whose in vitro-synthesized peptide had a molecular weight of 55,000, was achieved.",

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AU - Okada, Yoshiie

AU - Omura, Tsuneo

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AB - Total RNA was prepared from the free polysomes of bovine adrenal cortex and fractionated by high-performance gel filtration chromatography using a TSK-GEL G4000SW or G5000PW column. The former column gave better separation of 28 and 18 S rRNA than did the latter column when a suitable elution condition was selected. The separation of mRNAs by gel filtration chromatography was examined by in vitro translation of the fractionated RNA samples, and the fractionation of mRNAs roughly according to their sizes was confirmed. Significant enrichment of the mRNA of cytochrome P-450(SCC), whose in vitro-synthesized peptide had a molecular weight of 55,000, was achieved.

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Fractionation of mammalian tissue mRNAs by high-performance gel filtration chromatography

Abstract

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