Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization: Monoclonal antibody for a ginseng saponin

Noriko Fukuda; Hiroyuki Tanaka; Yukihiro Shoyama

doi:10.1023/A:1008162703957

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg₁ and its characterization: Monoclonal antibody for a ginseng saponin

Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rg₁ was produced by fusing sprenocytes immunized with a ginsenoside Rg₁-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Re. The full measuring range of the assay extends from 0.3 μg ml^-1 to 10 μg ml^-1 of ginsenoside Rg₁.

Original language	English
Pages (from-to)	197-204
Number of pages	8
Journal	Cytotechnology
Volume	34
Issue number	3
DOIs	https://doi.org/10.1023/A:1008162703957
Publication status	Published - 2000

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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title = "Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization: Monoclonal antibody for a ginseng saponin",

abstract = "The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rg1 was produced by fusing sprenocytes immunized with a ginsenoside Rg1-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Re. The full measuring range of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 of ginsenoside Rg1.",

author = "Noriko Fukuda and Hiroyuki Tanaka and Yukihiro Shoyama",

note = "Funding Information: This research was supported by the Grant-in-Aid for Scientific Research, Ministry of Education, Sciences and Culture (# 11470470, Japan), the Sasakawa Scientific Research Grant, the Shorai Foundation for Science and Technology and The Uehara Memorial Fundation. The authors are greateful to these financial supports.",

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language = "English",

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T2 - Monoclonal antibody for a ginseng saponin

AU - Fukuda, Noriko

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

N1 - Funding Information: This research was supported by the Grant-in-Aid for Scientific Research, Ministry of Education, Sciences and Culture (# 11470470, Japan), the Sasakawa Scientific Research Grant, the Shorai Foundation for Science and Technology and The Uehara Memorial Fundation. The authors are greateful to these financial supports.

PY - 2000

Y1 - 2000

N2 - The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rg1 was produced by fusing sprenocytes immunized with a ginsenoside Rg1-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Re. The full measuring range of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 of ginsenoside Rg1.

AB - The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rg1 was produced by fusing sprenocytes immunized with a ginsenoside Rg1-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Re. The full measuring range of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 of ginsenoside Rg1.

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Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg₁ and its characterization: Monoclonal antibody for a ginseng saponin

Abstract

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