Formation of a Tight Complex between Amphidinol 3 and Sterols in Lipid Bilayers Revealed by Short-Range Energy Transfer

Manami Hieda, Koya Tsujimura, Masanao Kinoshita, Nobuaki Matsumori

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The exploration of molecular recognition in lipid bilayers is still extremely difficult. In this report, we leveraged short-range energy transfer (ET) that enabled detection of close contacts within 1 nm distance, and applied it to the interaction of natural products with sterols in lipid bilayers. Amphidinol 3 (AM3), a polyhydroxypolyene metabolite from a marine dinoflagellate, possesses potent antifungal activity by forming membrane pores in a sterol-dependent manner. Although AM3 was shown to interact directly with membrane sterols, the mode of interaction is yet to be fully elucidated. Herein, we found that AM3 and cholestatrienol (CTL), a fluorescent sterol, can be an ET pair because the emission spectrum of the former overlaps with the excitation of the latter. We further confirmed that CTL exerts the sterol-dependent pore formation of AM3 as in the case of cholesterol. Then, titration using intermolecular ET in bilayers revealed that AM3 and CTL form a 1:1 complex with a dissociation constant of 1.4

Original languageEnglish
Pages (from-to)1753-1759
Number of pages7
JournalBulletin of the Chemical Society of Japan
Volume95
Issue number12
DOIs
Publication statusPublished - Dec 2022

All Science Journal Classification (ASJC) codes

  • General Chemistry

Fingerprint

Dive into the research topics of 'Formation of a Tight Complex between Amphidinol 3 and Sterols in Lipid Bilayers Revealed by Short-Range Energy Transfer'. Together they form a unique fingerprint.

Cite this