Formation of a monoclonal antibody against glycyrrhizin and development of an ELISA

Hiroyuki Tanaka; Yukihiro Shoyama

doi:10.1248/bpb.21.1391

Formation of a monoclonal antibody against glycyrrhizin and development of an ELISA

Hiroyuki Tanaka, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization-tof mass spectrometry. Hybridomas secreting monoclonal antibody against glycyrrhizin were produced by fusing splenocytes immunized with a glycyrrhizin-bovine serum albumin conjugate with the HAT-sensitive mouse myeloma cell line P3- X63-Ag8-653. A very small cross-reaction appeared with glycyrrhetic acid monoglucuronide and glycyrrhetic acid. The full measuring range of the assay extends from 20 ng ml^-1 to 200 ng ml^-1 of glycyrrhizin.

Original language	English
Pages (from-to)	1391-1393
Number of pages	3
Journal	Biological and Pharmaceutical Bulletin
Volume	21
Issue number	12
DOIs	https://doi.org/10.1248/bpb.21.1391
Publication status	Published - 1998

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmaceutical Science

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abstract = "The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization-tof mass spectrometry. Hybridomas secreting monoclonal antibody against glycyrrhizin were produced by fusing splenocytes immunized with a glycyrrhizin-bovine serum albumin conjugate with the HAT-sensitive mouse myeloma cell line P3- X63-Ag8-653. A very small cross-reaction appeared with glycyrrhetic acid monoglucuronide and glycyrrhetic acid. The full measuring range of the assay extends from 20 ng ml-1 to 200 ng ml-1 of glycyrrhizin.",

author = "Hiroyuki Tanaka and Yukihiro Shoyama",

year = "1998",

doi = "10.1248/bpb.21.1391",

language = "English",

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journal = "Biological and Pharmaceutical Bulletin",

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AB - The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization-tof mass spectrometry. Hybridomas secreting monoclonal antibody against glycyrrhizin were produced by fusing splenocytes immunized with a glycyrrhizin-bovine serum albumin conjugate with the HAT-sensitive mouse myeloma cell line P3- X63-Ag8-653. A very small cross-reaction appeared with glycyrrhetic acid monoglucuronide and glycyrrhetic acid. The full measuring range of the assay extends from 20 ng ml-1 to 200 ng ml-1 of glycyrrhizin.

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Formation of a monoclonal antibody against glycyrrhizin and development of an ELISA

Abstract

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