Fluorescent nanodiamond - A novel nanomaterial for in vivo applications

Nitin Mohan, Bailin Zhang, Cheng Chun Chang, Liling Yang, Chao Sheng Chen, Chia Yi Fang, Hsiao Han Hsieh, Chun Yi Cho, Yi Chun Wu, Jui Hsia Weng, Bon Chu Chung, Huan Cheng Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)


Fluorescent nanodiamonds (FNDs) with a size in the range of 10-100 nm have been produced by ion irradiation and annealing, and isolated by differential centrifugation. Single particle spectroscopic characterization with confocal fluorescence microscopy and fluorescence correlation spectroscopy indicates that they are photostable and useful as an alternative to far-red fluorescent proteins for bioimaging applications. We demonstrate the application by performing in vivo imaging of bare and bioconjugated FND particles (100 nm in diameter) in C. elegans and zebrafishes and exploring the interactions between this novel nanomaterial and the model organisms. Our results indicate that FNDs can be delivered to the embryos of both organisms by microinjection and eventually into the hatched larvae in the next generation. No deleterious effects have been observed for the carbon-based nanoparticles in vivo. The high fluorescence brightness, excellent photostability, and nontoxic nature of the nanomaterial have allowed longterm imaging and tracking of embryogenesis in the organisms.

Original languageEnglish
Title of host publicationCarbon Functional Interfaces
Number of pages11
Publication statusPublished - 2011
Externally publishedYes
Event2011 MRS Spring Meeting - San Francisco, CA, United States
Duration: Apr 25 2011Apr 29 2011

Publication series

NameMaterials Research Society Symposium Proceedings
ISSN (Print)0272-9172


Other2011 MRS Spring Meeting
Country/TerritoryUnited States
CitySan Francisco, CA

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering


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