TY - JOUR
T1 - Fluorescent differential display
T2 - Arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer
AU - Ito, Takashi
AU - Kito, Keiji
AU - Adati, Naoki
AU - Mitsui, Yuko
AU - Hagiwara, Hisashi
AU - Sakaki, Yoshiyuki
N1 - Funding Information:
Acknowledgements: We are grateful to S. Kohsaka (Natl. Inst. Neuroscience) for his generous gift of SH-SY5Y cells, K. Shiokawa (Univ. Tokyo) for advice on Xenopus embryology and encouragement, T. Ueda (Niigata Univ.) for mouse RNA samples, and N. Aoyama and M. Hirose for their excellent technical assistance. This work was partly supported by grants from Ministry of Education, Science and Culture, and from Science Technology Agency, Japan.
PY - 1994/9/5
Y1 - 1994/9/5
N2 - We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such 'Fluorescent Differential Display (FDD)' techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.
AB - We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such 'Fluorescent Differential Display (FDD)' techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.
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U2 - 10.1016/0014-5793(94)00867-1
DO - 10.1016/0014-5793(94)00867-1
M3 - Article
C2 - 7521850
AN - SCOPUS:0028041101
SN - 0014-5793
VL - 351
SP - 231
EP - 236
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -