Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor

Masayasu Taki; Mika Desaki; Akio Ojida; Shohei Iyoshi; Tasuku Hirayama; Itaru Hamachi; Yukio Yamamoto

doi:10.1021/ja803429z

Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor

Masayasu Taki, Mika Desaki, Akio Ojida, Shohei Iyoshi, Tasuku Hirayama, Itaru Hamachi, Yukio Yamamoto

Research output: Contribution to journal › Article › peer-review

195 Citations (Scopus)

Abstract

We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd²⁺-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd²⁺. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd²⁺ levels with the use of two excited wavelengths.

Original language	English
Pages (from-to)	12564-12565
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	130
Issue number	38
DOIs	https://doi.org/10.1021/ja803429z
Publication status	Published - Sept 24 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja803429z

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title = "Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor",

abstract = "We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths.",

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AU - Taki, Masayasu

AU - Desaki, Mika

AU - Ojida, Akio

AU - Iyoshi, Shohei

AU - Hirayama, Tasuku

AU - Hamachi, Itaru

AU - Yamamoto, Yukio

PY - 2008/9/24

Y1 - 2008/9/24

N2 - We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths.

AB - We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths.

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JF - Journal of the American Chemical Society

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Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor

Abstract

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